Developmental variation of the SUUR protein binding correlates with gene regulation and specific chromatin types in D-melanogaster
Developmental variation of the SUUR protein binding correlates with gene regulation and specific chromatin types in D-melanogaster
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DOI：
10.1007/s00412-013-0445-6
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发表时间：
2014-06-01
期刊：
影响因子：
1.6
通讯作者：
Belyakin, Stepan N.
中科院分区：
文献类型：
--
作者：
Maksimov, Daniil A.;Koryakov, Dmitry E.;Belyakin, Stepan N.
真核基因组被组织在大的染色质结构域中，以维持细胞中适当的基因活性。这些结构域可能允许或抑制潜在基因的转录。根据其蛋白质组成，果蝇细胞培养中的染色质最近被分为五种颜色编码状态。复制不足抑制蛋白(SUUR)被发现是存在于所有三种抑制染色质状态(黑色、蓝色和绿色)的主要成分，并且从活跃的黄色和红色染色质类型中被耗尽。在这里，我们讨论了作为抑制染色质类型的标记的SUUR结合的发育动力学问题。我们使用DAMID技术在幼虫唾液腺、脑和胚胎中建立了全基因组的SUUR结合谱，并将它们与已发表的果蝇KC细胞数据进行了配对比较和比较。发现不同样品之间的SUUR结合模式不同。SUUR结合的增加主要与局部基因抑制相关，提示异染色质的形成。SUUR结合的减少通常与组织特异性基因的激活相一致，这可能反映了向允许染色质状态的转变和对特定转录因子的可获得性的增加。在黑色、蓝色和红色染色质类型中，主要观察到伴随着潜在基因调控的SUUR结合可塑性。我们的结果为抑制染色质的发育动态提供了新的见解，并揭示了与染色质引导的基因表达调控的联系。
Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded states. Suppressor of Under-Replication (SUUR) protein was found to be the major component present in all three repressive chromatin states named BLACK, BLUE, and GREEN and to be depleted from the active YELLOW and RED chromatin types. Here, we addressed the question of developmental dynamics of SUUR binding as a marker of repressed chromatin types. We established genomewide SUUR binding profiles in larval salivary gland, brain, and embryos using DNA adenine methyltransferase identification (DamID) technique, performed their pairwise comparisons and comparisons with the published data from Drosophila Kc cells. SUUR binding pattern was found to vary between the samples. Increase in SUUR binding predominantly correlated with local gene repression suggesting heterochromatin formation. Reduction in SUUR binding often coincided with activation of tissue-specific genes probably reflecting the transition to permissive chromatin state and increase in accessibility to specific transcription factors. SUUR binding plasticity accompanied by the regulation of the underlying genes was mainly observed in BLACK, BLUE, and RED chromatin types. Our results provide novel insight into the developmental dynamics of repressive chromatin and reveal a link to the chromatin-guided regulation of gene expression.