Protease production during growth and autolysis of submerged Metarhizium anisopliae cultures
Protease production during growth and autolysis of submerged Metarhizium anisopliae cultures
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水下金龟子绿僵菌培养物生长和自溶过程中蛋白酶的产生
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
C. Messias
中科院分区：
文献类型：
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作者：
G. U. Braga;R. Destéfano;C. Messias
本文研究了两株昆虫病原半知真菌金龟子绿僵菌（Metarhizium anisopliae var.）在含有酪蛋白或葡萄糖作为碳源的培养基中评价绿僵菌。测定了各菌株的经济系数和自溶度等参数。在蛋白酶诱导条件下（在酪蛋白作为唯一碳源和氮源的存在下），在培养基上的培养物的整个生长和自溶阶段测定蛋白酶产生。该真菌显示出以比葡萄糖更有效的方式利用酪蛋白作为碳/能源。在这两种生长条件下，菌株的自溶都很强烈，达到干物质的62.7%，并在外源碳源耗尽后不久开始。两种菌株的蛋白水解活性之间的关系差异很大（在生长的第5天最大值为19.78，在生长的第16天最小值为2.03），清楚地表明生长曲线之间的差异和酶产生动力学的差异可能决定性地影响蛋白酶菌株选择的过程生产
The growth and autolysis of two strains of the entomopathogenic deuteromycete fungus Metarhizium anisopliae var. anisopliae were evaluated in medium containing casein or glucose as carbon source. Parameters such as economic coefficient and degree of autolysis were determined for each strain. Protease production was determined throughout the growth and autolysis phases of the cultures on medium under conditions of protease induction (in the presence of casein as sole source of carbon and nitrogen). The fungus was shown to utilize casein as a carbon/energy source in a more efficient manner than glucose. The autolysis shown by the strains was intense under both types of growth conditions, reaching up to 62.7% of the dry mass produced and started soon after the depletion of the exogenous carbon source. The relationship between the proteolytic activities of the two strains evaluated varied significantly (a maximum of 19.78 on the 5th day and a minimum of 2.03 on the 16th day of growth) during the various growth and autolysis phases, clearly showing that the difference between the growth curves and the difference in the kinetics of enzyme production may decisively affect the process of strain selection for protease production.