The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits ENaC through an increase in the intracellular Cl- concentration
The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits ENaC through an increase in the intracellular Cl- concentration
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DOI：
10.1093/embo-reports/kve232
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发表时间：
2001-11-01
期刊：
影响因子：
7.7
通讯作者：
Kunzelmann, K
中科院分区：
文献类型：
--
作者：
König, J;Schreiber, R;Kunzelmann, K
根据对上皮细胞和过表达重组细胞的研究，CFTR Cl-通道的激活抑制上皮Na+通道（ENaC）。在这里，我们表明，ENaC抑制在刺激的囊性纤维化跨膜电导调节器（CFTR）在非洲爪蟾卵母细胞，独立的实验设置和全细胞电流的大小。在较高的CFTR Cl-电流下，ENaC的抑制增强。与CFTR类似，ClC-0 Cl-电流也抑制ENaC，以及部分透化卵母细胞中的高细胞外Na+和Cl-。因此，ENaC的抑制不是CFTR特异性的，似乎是由Cl-介导的。
Activation of the CFTR Cl- channel inhibits epithelial Na+ channels (ENaC), according to studies on epithelial cells and overexpressing recombinant cells. Here we demonstrate that ENaC is inhibited during stimulation of the cystic fibrosis trans-membrane conductance regulator (CFTR) in Xenopus oocytes, independent of the experimental set-up and the magnitude of the whole-cell current. Inhibition of ENaC is augmented at higher CFTR Cl- currents. Similar to CFTR, ClC-0 Cl- currents also inhibit ENaC, as well as high extracellular Na+ and Cl- in partially permeabilized oocytes. Thus, inhibition of ENaC is not specific to CFTR and seems to be mediated by Cl-.