Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells
Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells
复制标题
基于固相的活体真核细胞染色质结构和转录因子占用的快速自动分析
作者：
Richard M. Ingram;H. Tagoh;A. Riggs;C. Bonifer
转录因子、染色质组分和染色质修饰活性与包括癌症在内的许多疾病有关。然而，这些因素的改变影响特定细胞类型的表观遗传型的手段尚不清楚。限制进展的一个问题是真核基因的调控区域有时延伸到DNA的大区域。为了改善如此大区域的染色质结构-功能分析，我们开发了一种自动化的，相对简单的程序，使用磁珠和毛细管测序仪进行连接介导pcr （LM-PCR）。我们表明，该程序可用于快速检查染色质精细结构，核小体定位以及细胞分化过程中转录因子结合位点占用的变化。
Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly understood. One problem that limits progress is that regulatory regions of eukaryotic genes sometimes extend over large regions of DNA. To improve chromatin structure–function analysis over such large regions, we have developed an automated, relatively simple procedure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR). We show that the procedure can be used for the rapid examination of chromatin fine-structure, nucleosome positioning as well as changes in transcription factor binding-site occupancy during cellular differentiation.
通过连接介导和末端转移酶介导的聚合酶链反应进行染色质结构分析。
影响因子：
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作者：
Pfeifer,GP;Chen,HH;Komura,J;Riggs,AD
通讯作者：
Riggs,AD
影响因子：
56.9
作者：
MUELLER, PR;WOLD, B
通讯作者：
WOLD, B