P2X7 receptors enhance glutamate release in hippocampal hilar neurons
P2X7 receptors enhance glutamate release in hippocampal hilar neurons
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DOI：
10.1097/wnr.0b013e32833d9142
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发表时间：
2010-09-15
期刊：
影响因子：
1.7
通讯作者：
Jang, Il-Sung
中科院分区：
文献类型：
--
作者：
Cho, Jin-Hwa;Choi, In-Sun;Jang, Il-Sung
我们研究了P2 X7受体激动剂2 '-3'-O-（4-苯甲酰基苯甲酰基）-腺苷-5 '-三磷酸（Bz-ATP）对机械分离的未成熟肺门神经元连接的神经末梢的动作电位非依赖性谷氨酸释放的影响。Bz-ATP增加自发兴奋性突触后电流（sEPSC）频率，这种作用可被P2 X7受体拮抗剂亮蓝G阻断，表明P2 X7受体介导Bz-ATP对sEPSC的易化作用。在大多数测试的门神经元中，Bz-ATP诱导的sEPSC频率的增加被河豚毒素或Cd ~（2+）阻断，表明P2 X7受体的激活导致突触前去极化。P2 X7受体介导的谷氨酸释放的易化作用将调节门神经元的兴奋性，并最终对海马介导的病理生理功能产生广泛影响。NeuroReport 21：865-870（C）2010年威科健康垂直酒吧Lippincott威廉姆斯&威尔金斯。
We examined the effect of 2'-3'-O-(4-benzoylbenzoyl)-adenosine-5'-triphosphate (Bz-ATP), a P2X7 receptor agonist, on action potential-independent glutamate release from nerve terminals attached to mechanically isolated immature hilar neurons. Bz-ATP increased spontaneous excitatory postsynaptic current (sEPSC) frequency, and this effect was blocked by Brilliant blue G, a P2X7 receptor antagonist, suggesting that P2X7 receptors mediate the facilitatory action of Bz-ATP on sEPSCs. In most of hilar neurons tested, the Bz-ATP-induced increase in sEPSC frequency was blocked by tetrodotoxin or Cd2+, suggesting that the activation of P2X7 receptors leads to a presynaptic depolarization. The P2X7 receptor-mediated facilitation of glutamate release would modulate the excitability of hilar neurons, and eventually have a broad impact on the pathophysiological functions mediated by the hippocampus. NeuroReport 21: 865-870 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.