The Soybean-Specific Maturity Gene E1 Family of Floral Repressors Controls Night-Break Responses through Down-Regulation of FLOWERING LOCUS T Orthologs
The Soybean-Specific Maturity Gene E1 Family of Floral Repressors Controls Night-Break Responses through Down-Regulation of FLOWERING LOCUS T Orthologs
复制标题
大豆特异性成熟基因 E1 花抑制基因家族通过下调 FLOWERING LOCUS T 直系同源物来控制夜间反应。
DOI：
10.1104/pp.15.00763
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发表时间：
2015-08-01
期刊：
影响因子：
7.4
通讯作者：
Abe, Jun
中科院分区：
文献类型：
--
作者：
Xu, Meilan;Yamagishi, Noriko;Abe, Jun
光周期性是植物对光的敏感性的节律性变化，有助于植物根据日照长度的季节变化调整开花时间，适应不同纬度的生长条件。为了揭示兼性短日植物大豆（Glycine max）光周期性的分子基础，我们分析了成熟基因E1家族和两个FLOWARNLOCUS T（FT）直系同源基因（FT 2a和FT 5a）的转录谱。FT 2a和FT 5a的阻遏物E1及其两个同源物E1-like-a（E1 La）和E1 Lb在长日照下表现出两个表达峰。使用两种不同的方法（实验与光和暗阶段之间的过渡和夜间休息实验），我们发现，E1家族基因的表达只在光周期和他们的诱导后，黎明在漫长的日子需要一段时间的光黄昏前的前一天。在缺乏E1基因的栽培品种丰菇中，病毒诱导的E1 La和E1 Lb的沉默上调了FT 2a和FT 5a的表达，并导致提早开花。因此，E1、E1 La和E1 Lb在开花中的功能相似。光对E1和E1 L表达的调节受编码光敏色素A蛋白的E3和E4的控制。我们的数据表明，光敏色素A介导的转录诱导E1及其同系物的光在大豆开花的光周期诱导中起着至关重要的作用。
Photoperiodism is a rhythmic change of sensitivity to light, which helps plants to adjust flowering time according to seasonal changes in daylength and to adapt to growing conditions at various latitudes. To reveal the molecular basis of photoperiodism in soybean (Glycine max), a facultative short-day plant, we analyzed the transcriptional profiles of the maturity gene E1 family and two FLOWERING LOCUS T (FT) orthologs (FT2a and FT5a). E1, a repressor for FT2a and FT5a, and its two homologs, E1-like-a (E1La) and E1Lb, exhibited two peaks of expression in long days. Using two different approaches (experiments with transition between light and dark phases and night-break experiments), we revealed that the E1 family genes were expressed only during light periods and that their induction after dawn in long days required a period of light before dusk the previous day. In the cultivar Toyomusume, which lacks the E1 gene, virus-induced silencing of E1La and E1Lb up-regulated the expression of FT2a and FT5a and led to early flowering. Therefore, E1, E1La, and E1Lb function similarly in flowering. Regulation of E1 and E1L expression by light was under the control of E3 and E4, which encode phytochrome A proteins. Our data suggest that phytochrome A-mediated transcriptional induction of E1 and its homologs by light plays a critical role in photoperiodic induction of flowering in soybean.