Late stage infection in sleeping sickness.
Late stage infection in sleeping sickness.
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DOI：
10.1371/journal.pone.0034304
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Duszenko M
中科院分区：
文献类型：
--
作者：
Wolburg H;Mogk S;Acker S;Frey C;Meinert M;Schönfeld C;Lazarus M;Urade Y;Kubata BK;Duszenko M
在世纪之交，锥虫被确定为昏睡病的病原体，并描述了它们在晚期昏睡病患者的脑脊液中的存在。然而，到目前为止，还没有确切的证据证明寄生虫是如何到达大脑的。分析电子显微镜下制备的啮齿动物的大脑感染后20多天，我们在这里提出了确凿的证据表明，寄生虫首先进入大脑通过脉络丛，从那里他们穿透上皮细胞层到达脑室系统。贴壁，在血管外的实质内未观察到锥虫。我们还表明，脑感染依赖于细长锥虫的形成，脑脊液以及脉络丛的基质是锥虫生存的不利环境，锥虫通过蛛网膜下腔进入软脑膜空间，包括Virchow-Robin空间，以逃避降解。我们的数据表明，锥虫并不打算在大脑中定居，而是居住在胶质界膜附近或内部，从那里它们可以重新填充血管并扰乱睡眠/觉醒周期。
At the turn of the 19th century, trypanosomes were identified as the causative agent of sleeping sickness and their presence within the cerebrospinal fluid of late stage sleeping sickness patients was described. However, no definitive proof of how the parasites reach the brain has been presented so far. Analyzing electron micrographs prepared from rodent brains more than 20 days after infection, we present here conclusive evidence that the parasites first enter the brain via the choroid plexus from where they penetrate the epithelial cell layer to reach the ventricular system. Adversely, no trypanosomes were observed within the parenchyma outside blood vessels. We also show that brain infection depends on the formation of long slender trypanosomes and that the cerebrospinal fluid as well as the stroma of the choroid plexus is a hostile environment for the survival of trypanosomes, which enter the pial space including the Virchow-Robin space via the subarachnoid space to escape degradation. Our data suggest that trypanosomes do not intend to colonize the brain but reside near or within the glia limitans, from where they can re-populate blood vessels and disrupt the sleep wake cycles.
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影响因子：
--
作者：
Castellant, A
通讯作者：
Castellant, A
影响因子：
12.4
作者：
Figarella, K;Rawer, M;Duszenko, M
通讯作者：
Duszenko, M
DOI：
10.1016/0035-9203(80)90184-4
发表时间：
1980-01-01
影响因子：
2.2
作者：
GREENWOOD, BM;WHITTLE, HC
通讯作者：
WHITTLE, HC
影响因子：
2.2
作者：
BLACK, SJ;HEWETT, RS;SENDASHONGA, CN
通讯作者：
SENDASHONGA, CN
影响因子：
20.3
作者：
Lyck, R;Reiss, Y;Engelhardt, B
通讯作者：
Engelhardt, B