ACUTE EFFECTS OF NEAR ULTRAVIOLET AND VISIBLE-LIGHT ON THE CUTANEOUS ANTIOXIDANT DEFENSE SYSTEM
ACUTE EFFECTS OF NEAR ULTRAVIOLET AND VISIBLE-LIGHT ON THE CUTANEOUS ANTIOXIDANT DEFENSE SYSTEM
复制标题
DOI：
10.1111/j.1751-1097.1989.tb02904.x
复制
发表时间：
1989-12-01
影响因子：
3.3
通讯作者：
PACKER, L
中科院分区：
文献类型：
--
作者：
FUCHS, J;HUFLEJT, ME;PACKER, L
活性氧被认为在皮肤病理学中起重要作用。酶和非酶抗氧化剂可以防止氧化损伤，但可能被强烈的促氧化刺激所克服。在无毛小鼠中，在照射后立即检查了单次暴露于近紫外线（UVA）/可见光辐射（> 320 nm）对各种皮肤抗氧化剂的急性影响。观察到皮肤过氧化氢酶和谷胱甘肽还原酶活性受损。超氧化物歧化酶和谷胱甘肽过氧化物酶没有显着影响。过氧化氢酶的抑制可能使皮肤更容易受到过氧化氢及其反应产物（如羟基自由基）的破坏作用。谷胱甘肽还原酶活性的部分降低并不伴随着照射后立即发生的还原型/氧化型谷胱甘肽水平的变化。皮肤生育酚、泛醇+泛醌9和抗坏血酸水平有趋势（统计学上不显着）下降，这表明直接光破坏或被光氧化应激反应产物消耗。近紫外线/可见光对皮肤抗氧化防御系统的这种部分损害表明，皮肤中最敏感的成分是过氧化氢酶，这表明可能的药物干预。
Reactive oxygen species are considered to play an important role in cutaneous pathology. Enzymic and non-enzymic antioxidants can prevent oxidative damage but may be overcome by strong pro-oxidative stimuli. The acute effect of a single exposure to near ultraviolet (UVA)/visible radiation (> 320 nm) on various skin antioxidants was examined in hairless mice immediately after irradiation. Impairment of cutaneous catalase and glutathione reductase activity was observed. Superoxide dismutase and glutathione peroxidase were not significantly influenced. Inhibition of catalase may render skin more susceptible to the damaging effects of hydrogen peroxide and its reaction products such as the hydroxyl radical. Partially diminished glutathione reductase activity is not accompanied by a change in reduced/oxidized glutathione level immediately after irradiation. There was a tendential (not statistically significant) decrease in cutaneous tocopherol, ubiquinol + ubiquinone 9 and ascorbic acid levels, either indicating direct photodestruction or consumption by reaction products of photooxidative stress. This partial impairment of the cutaneous antioxidant defense system by near ultraviolet/visible light, showing that the most susceptible component in skin is catalase, suggests possible pharmacological interventions.