Dietary fiber prevents obesity-related liver lipotoxicity by modulating sterol-regulatory element binding protein pathway in C57BL/6J mice fed a high-fat/cholesterol diet.
Dietary fiber prevents obesity-related liver lipotoxicity by modulating sterol-regulatory element binding protein pathway in C57BL/6J mice fed a high-fat/cholesterol diet.
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DOI：
10.1038/srep15256
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发表时间：
2015-10-29
影响因子：
4.6
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Han S;Jiao J;Zhang W;Xu J;Wan Z;Zhang W;Gao X;Qin L
摄入足够的膳食纤维已被证明对代谢和心血管有益，但分子机制仍然有限。本研究旨在探讨谷类膳食纤维对喂饲高脂/胆固醇(HFC)饲料的C57BL/6J小鼠肥胖相关肝脂毒性的影响及其机制。将48只成年雄性C57BL/6J小鼠随机分为对照组和高脂/胆固醇(HFC)饲料，分别添加或不添加燕麦纤维和麸皮纤维，共24周。我们的结果显示，与HFC饮食相比，喂食燕麦或麦皮纤维的小鼠体重增加、血脂水平和胰岛素抵抗都较低。这两种谷物膳食纤维有效地降低了目标组织中固醇调节元件结合蛋白-1和参与脂肪形成的关键因素，包括脂肪酸合成酶和乙酰辅酶A羧基酶的蛋白表达。在分子水平上，这两种谷类膳食纤维增加了目标组织中过氧化体增殖物激活受体α和伽马、肝脏X受体α和ATP结合盒转运蛋白A1的蛋白表达。我们的研究结果表明，补充谷类膳食纤维可以消除喂食HFC饮食的C57BL/6J小鼠肥胖相关的肝脏脂毒性和血脂异常。此外，燕麦纤维在使这些代谢紊乱和病理特征正常化方面比小麦麸皮纤维的功效更大。
Adequate intake of dietary fibers has proven metabolic and cardiovascular benefits, molecular mechanisms remain still limited. This study was aimed to investigate the effects of cereal dietary fiber on obesity-related liver lipotoxicity in C57BL/6J mice fed a high-fat/cholesterol (HFC) diet and underlying mechanism. Forty-eight adult male C57BL/6J mice were randomly given a reference chow diet, or a high fat/choleserol (HFC) diet supplemented with or without oat fiber or wheat bran fiber for 24 weeks. Our results showed mice fed oat or wheat bran fiber exhibtied lower weight gain, lipid profiles and insulin resistance, compared with HFC diet. The two cereal dietary fibers potently decreased protein expressions of sterol regulatory element binding protein-1 and key factors involved in lipogenesis, including fatty acid synthase and acetyl-CoA carboxylase in target tissues. At molecular level, the two cereal dietary fibers augmented protein expressions of peroxisome proliferator-activated receptor alpha and gamma, liver X receptor alpha, and ATP-binding cassette transporter A1 in target tissues. Our findings indicated that cereal dietary fiber supplementation abrogated obesity-related liver lipotoxicity and dyslipidemia in C57BL/6J mice fed a HFC diet. In addition, the efficacy of oat fiber is greater than wheat bran fiber in normalizing these metabolic disorders and pathological profiles.