Elucidation of spermidine interaction with nucleotide ATP by multiple NMR techniques.
Elucidation of spermidine interaction with nucleotide ATP by multiple NMR techniques.
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通过多种 NMR 技术阐明亚精胺与核苷酸 ATP 的相互作用。
DOI：
10.1002/mrc.2554
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Olubajo,Olarongbe
中科院分区：
文献类型：
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作者：
Song,Zhiyan;Parker,KariJ;Enoh,Idorenyin;Zhao,Hua;Olubajo,Olarongbe
Interaction of polyamines with nucleotides plays a key role in many biological processes. Here we use multiple NMR techniques to characterize interaction of spermidine with adenosine 5′‐triphosphate (ATP). Two‐dimensional1H‐15N spectra obtained from gs‐HMBC experiments at varied pH show significant shift of N‐1 peak around pH 2.0–7.0 range, suggesting that spermidine binds to N‐1 site of ATP base. The binding facilitates N‐1 deprotonation, shifting its pKafrom 4.3 to 3.4. By correlating15N and31P chemical shift data, it is clear that spermidine is capable of concurrently binding to ATP base and phosphate sites around pH 4.0–7.0. The self‐diffusion constants derived from1H PFG‐diffusion measurements provide evidence that binding of spermidine to ATP is in 1:1 ratio, and pH variations do not induce significant nucleotide self‐association in our samples.31P spectral analysis suggests that at neutral pH, Mg2+ion competes with spermidine and shows stronger binding to ATP phosphates. From31P kinetic measurements of myosin‐catalyzed ATP hydrolysis, it is found that binding of spermidine affects the stability and reactivity of ATP. These NMR results are important for advancing the studies on nucleotide–polyamine interaction and its impact on nucleotide structures and activities under varied conditions. Copyright © 2009 John Wiley & Sons, Ltd.
