Streptomyces karpasiensis sp. nov., isolated from soil.
Streptomyces karpasiensis sp. nov., isolated from soil.
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DOI：
10.1099/ijs.0.056275-0
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发表时间：
2014-03
影响因子：
2.8
通讯作者：
Aysel Veyisoğlu;D. Tatar;Demet Cetin;K. Guven;N. Şahin
中科院分区：
文献类型：
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作者：
Aysel Veyisoğlu;D. Tatar;Demet Cetin;K. Guven;N. Şahin
从塞浦路斯北方马古萨的卡帕斯国家公园的土壤中分离到一株放线菌，命名为K413（T），并对其进行了分类学鉴定。发现该分离物具有与链霉菌属成员相关的化学和形态学特性。系统发育分析的基础上，几乎完整的16 S rRNA基因序列表明，该分离物是密切相关的链霉菌属的成员，并显示出形成一个独特的系统发育树链霉菌。菌株K413（T）与海洋链霉菌DSM 41968（T）的亲缘关系最近（98.01%）。与链霉菌属其他菌株的序列相似性均在98.0%以下。新菌株的细胞壁含有11-二氨基庚二酸。主要的甲基萘醌是MK-9（H8）（45.0%）。检测到的极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰单甲基乙醇胺和磷脂酰肌醇。主要脂肪酸为anteiso-C15：0、iso-C16：0和anteiso-C17：0。根据16 S rRNA基因序列分析、DNA-DNA亲缘关系、表型特征和化学分类学数据，菌株K413（T）代表链霉菌属的一个新种，并建议将其命名为Streptomyceskarpasiensissp. nov.。模式菌株为K413（T）（= KCTC 29096（T）= DSM 42068（T））。
A novel actinobacteria, designated strain K413(T), was isolated from soil collected from Karpaz National Park, Magusa, Northern Cyprus, and characterized to determine its taxonomic position. The isolate was found to have chemical and morphological properties associated with members of the genus Streptomyces. Phylogenetic analyses based on almost-complete 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus Streptomyces, and was shown to form a distinct phyletic line in the Streptomyces phylogenetic tree. Strain K413(T) was most closely related to Streptomyces marinus DSM 41968(T) (98.01%). Sequence similarities with other strains of the genus Streptomyces were below 98.0%. The cell wall of the novel strain contained ll-diaminopimelic acid. The predominant menaquinone was MK-9(H8) (45.0%). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylinositol. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Based on 16S rRNA gene sequence analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain K413(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces karpasiensis sp. nov. is proposed. The type strain is K413(T) ( = KCTC 29096(T) = DSM 42068(T)).