Antigen-antibody complexes generate Lyt 1 inducers of suppressor cells.
Antigen-antibody complexes generate Lyt 1 inducers of suppressor cells.
复制标题
抗原-抗体复合物产生抑制细胞的 Lyt 1 诱导剂。
作者：
V. Rao;J. Bennett;F. Shen;R. Gershon;M. Mitchell
用H-2d肿瘤（L1210）的KCl提取物与L1210抗体一起预处理H-2b小鼠使得小鼠不能对活的L1210肿瘤细胞产生有效的免疫应答。这种特异性免疫抑制与巨噬细胞功能抑制有关，可用于定量抑制水平。用抗原抗体复合物预处理的小鼠的脾脏或胸腺的Lyt 1细胞（但不是Lyt 2细胞）可以将抑制状态过继转移到正常小鼠。转移抑制的Lyt 1细胞对低剂量（20 mg/kg）环磷酰胺（Cy）具有抗性，但需要激活Cy敏感的前体和/或放大细胞。一旦激活，它们需要正常受体中的Cy-sensitive Lyt 1 2 3受体细胞来有效抑制过继宿主的巨噬细胞。我们的研究结果表明，免疫复合物与Cy-sensitive T细胞协同产生抗Cy-Lyt 1抑制诱导剂。这些反过来激活正常的胸腺或外周Lyt 1 2 3受体细胞，导致产生效应抑制T细胞。这些抑制细胞很可能是我们在体内观察到的巨噬细胞抑制的近因。
Pretreatment of H-2b mice with KCl-extracts of an H-2d tumor (L1210) together with L1210 antibody rendered the mice incapable of mounting an effective immune response to viable L1210 tumor cells. This specific immunosuppression was associated with inhibition of macrophage functions, which could be used to quantitate the level of suppression. Lyt 1 cells (but not Lyt 2 cells) from either the spleen or thymus of mice pretreated with antigen-antibody complexes could adoptively transfer the suppressed state to normal mice. The Lyt 1 cells that transferred the suppression were resistant to low (20 mg/kg) doses of cyclophosphamide (Cy) but required a Cy-sensitive precursor and/or amplifier cell to be activated. Once activated, they required a Cy-sensitive Lyt 1 2 3 acceptor cell in the normal recipient to effectively suppress the adoptive host's macrophages. Our results indicative that immune complexes in concert with a Cy-sensitive T cell generate Cy-resistant Lyt 1 inducers of suppression. These in turn activate normal thymic or peripheral Lyt 1 2 3 acceptor cells, resulting in the generation of effector suppressor T cells. These suppressor cells are most likely the proximate cause of the inhibition of macrophages we have observed in vivo.