Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines.
Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines.
复制标题
对单个轴突、树突和棘的各个区域的亚毫秒膜电位变化进行成像。
DOI：
10.1007/978-3-319-17641-3_3
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发表时间：
2015
影响因子：
--
通讯作者：
Zecevic,Dejan
中科院分区：
文献类型：
--
作者：
Popovic,Marko;Vogt,Kaspar;Holthoff,Knut;Konnerth,Arthur;Salzberg,BrianM;Grinvald,Amiram;Antic,SrdjanD;Canepari,Marco;Zecevic,Dejan
神经元网络分析中的一个核心问题是单个神经元之间的相互作用如何产生行为和行为修改。这项任务关键取决于信号是如何被作为复杂操作单元的单个神经细胞整合的。决定任何神经元的输入输出功能的分支神经元过程的区域电特性是非常复杂的，动态的，并且在一般情况下，在没有详细测量的情况下是不可能预测的。为了获得这样的测量，理想情况下，人们希望能够在多个部位监测亚阈值事件，因为它们从起源部位（远端树突上的突触接触）行进并在特定位置处求和以影响动作电位起始。最近，利用细胞内电压敏感染料高分辨率多位点记录膜电位变化来进行这种类型的测量成为可能。本章回顾了电压敏感染料记录单个神经元方法的发展和基础。目前，这种方法允许监测来自树突树的所有部分以及来自轴突侧支和个体树突棘的膜电位瞬变。
A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on how exactly signals are integrated by individual nerve cells functioning as complex operational units. Regional electrical properties of branching neuronal processes which determine the input-output function of any neuron are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor, at multiple sites, subthreshold events as they travel from the sites of origin (synaptic contacts on distal dendrites) and summate at particular locations to influence action potential initiation. It became possible recently to carry out this type of measurement using high-resolution multisite recording of membrane potential changes with intracellular voltage-sensitive dyes. This chapter reviews the development and foundation of the method of voltage-sensitive dye recording from individual neurons. Presently, this approach allows monitoring membrane potential transients from all parts of the dendritic tree as well as from axon collaterals and individual dendritic spines.