Prognostic significance of Akt, phospho-Akt and BAD expression in primary breast cancer
Prognostic significance of Akt, phospho-Akt and BAD expression in primary breast cancer
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DOI：
10.1016/j.ejca.2008.11.044
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发表时间：
2009-03-01
影响因子：
8.4
通讯作者：
Dobson, Pauline R. M.
中科院分区：
文献类型：
--
作者：
Al-Bazz, Yousif O.;Underwood, James C. E.;Dobson, Pauline R. M.
据报道，乳腺癌中的凋亡标志物具有预后意义。本研究的目的是评估Akt、磷酸化Akt和BAD在乳腺癌患者原发肿瘤中的表达的预后价值。磷酸化Akt的表达与绝经状态、淋巴结受累或肿瘤大小无关，尽管磷酸化Akt与雌激素受体状态和肿瘤分级之间存在显著相关性。未发现磷酸化Akt和BAD之间存在关联。然而，发现Akt和BAD之间存在显著相关性。Akt和磷酸化Akt的表达与无病生存期（DFS）或总生存期（OS）均无关。相反，在冷冻切片和石蜡切片中，BAD免疫染色与肿瘤增大以及雌激素受体（ER）免疫染色显著相关。除了细胞质和细胞核染色外，BAD的表达似乎还呈核仁状。对冷冻切片和石蜡切片上免疫组化染色的比较显示，Akt和BAD的免疫反应性具有合理的一致性。然而，研究结果首次表明，BAD的高表达与良好的预后相关，但不是一个独立的预后因素。总之，这些结果可为理解Akt、磷酸化Akt和BAD的表达如何影响浸润性乳腺癌的预后提供依据。（C）2008爱思唯尔有限公司。保留所有权利。
Apoptotic markers in breast cancer are reported to have prognostic significance. The aim of this study was to assess the prognostic value of Akt, phospho-Akt and BAD expression in primary tumours from breast cancer patients. Expression of phospho-Akt did not correlate with menopausal status, nodal involvement or tumour size, although there was a significant correlation between phospho-Akt and oestrogen receptor status and tumour grade. No association was found between phospho-Akt and BAD. However, a significant correlation was found between Akt and BAD. Akt and phospho-Akt expression did not correlate with either disease-free survival (DFS) or overall survival (OS). Conversely, BAD immunostaining correlated significantly with increasing tumour size and with oestrogen receptor (ER) immunostaining in both frozen and paraffin sections. Expression of BAD appeared to be nucleolar in addition to its cytoplasmic and nuclear staining. Comparison of immunohistochemical staining on frozen sections and paraffin sections showed a reasonable concordance in Akt and BAD immunoreactivity. However, the results showed for the first time that strong BAD expression is related to a favourable prognosis but is not an independent prognostic factor. in conclusion, these results could provide the basis for understanding how Akt, phospho-Akt and BAD expression contributes to the prognosis of invasive breast cancer. (C) 2008 Elsevier Ltd. All rights reserved.