Osteopontin exacerbates the progression of experimental autoimmune myasthenia gravis by affecting the differentiation of T cell subsets
Osteopontin exacerbates the progression of experimental autoimmune myasthenia gravis by affecting the differentiation of T cell subsets
复制标题
骨桥蛋白通过影响 T 细胞亚群的分化加剧实验性自身免疫性重症肌无力的进展
DOI：
10.1016/j.intimp.2020.106335
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Wang Jinghua
中科院分区：
文献类型：
--
作者：
Zhao Jiarui;Jing Jia;Zhao Wei;Li Xinrong;Hou Lixuan;Zheng Chunfeng;Kong Qingfei;Li Wenjin;Yao Xiuhua;Chang Lulu;Li Hulun;Mu Lili;Wang Guangyou;Wang Jinghua
骨桥蛋白（OPN）是一种多功能的细胞外基质磷蛋白，具有特殊而复杂的结构，参与多种生理和病理活动。骨桥蛋白在许多疾病中的作用机制已被证实，但骨桥蛋白在重症肌无力（MG）中的作用尚不清楚。在本研究中，我们在体外重组大鼠OPN蛋白，并使用实验性自身免疫性重症肌无力（EAMG）大鼠模型评估OPN如何影响自身免疫的发展。结果表明，血清中OPN浓度上调。EAMG模型脾细胞中mRNA和蛋白水平均升高。OPN体外处理可明显促进Th1细胞的分化，抑制Treg细胞的分化。腹腔注射OPN提示EAMG发病早，病情重。这种效应伴随着Th1细胞百分比的增加。结论：OPN可能通过促进Th1细胞的分化和抑制Treg细胞的分化而加重EAMG的发病。
Osteopontin (OPN) is a multifunctional extracellular matrix phosphoprotein that has a specific and complicated structure, and contributes to numerous physiological and pathological activities. The mechanism of OPN in many diseases has been confirmed; however, the role of OPN in myasthenia gravis (MG) remains unclear. In this study, we recombined rat OPN proteinin vitro, and assessed how OPN affects the development of autoimmunity using an experimental autoimmune myasthenia gravis (EAMG) rat model. The results showed that the concentration of OPN in serum was up-regulated. Both mRNA and protein levels in splenocytes increased in the EAMG model. OPN treatmentin vitrostrongly promoted the differentiation of Th1 cells, and inhibited the differentiation of Treg cells. Intraperitoneal injection of OPN revealed the early incidence of EAMG, and more serious disease. This effect was accompanied by an increased percentage of Th1 cells. In conclusion, OPN likely exacerbates the pathogenesis of EAMG by promoting the differentiation of Th1 cells and inhibiting the differentiation of Treg cells.