Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing*
Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing*
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DOI：
10.1074/jbc.m116.753046
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发表时间：
2016-10
期刊：
影响因子：
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通讯作者：
Ziqiang Wang;Qing Liu;Jinhua Lu;Ping Fan;W. Xie;W. Qiu;Fan Wang;Guangnan Hu;Yaou Zhang
中科院分区：
文献类型：
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作者：
Ziqiang Wang;Qing Liu;Jinhua Lu;Ping Fan;W. Xie;W. Qiu;Fan Wang;Guangnan Hu;Yaou Zhang
一旦进入宿主细胞，1型单纯疱疹病毒（HSV-1）就会招募一系列宿主细胞因子来促进其生命周期。在这里，我们证明，丝氨酸/丝氨酸丰富的剪接因子2（SRSF 2），这是一个重要组成部分的剪接斑点，介导的HSV-1复制通过调节病毒基因表达在转录和转录后水平。我们的研究结果表明，SRSF 2的功能作为一个转录激活因子，直接结合感染细胞多肽0（ICP 0），感染细胞多肽27（ICP 27），和胸苷激酶启动子。此外，SRSF 2通过识别ICP 0外显子3中的结合位点参与ICP 0前mRNA剪接。这些发现提供了深入了解SRSF 2在HSV-1复制和基因表达中的功能。
Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.