Collective Space-Sensing Coordinates Pattern Scaling in Engineered Bacteria.
Collective Space-Sensing Coordinates Pattern Scaling in Engineered Bacteria.
复制标题
集体空间感应在工程细菌中协调图案缩放。
DOI：
10.1016/j.cell.2016.03.006
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发表时间：
2016-04-21
期刊：
影响因子：
64.5
通讯作者：
You L
中科院分区：
文献类型：
--
作者：
Cao Y;Ryser MD;Payne S;Li B;Rao CV;You L
尺度不变性是指维持发育器官大小与身体大小的恒定比例。虽然常见，但其潜在机制仍然知之甚少。在这里，我们研究了工程E。能够在菌落中形成自组织的核-环模式。我们发现，环的宽度表现出完美的规模不变性的殖民地的大小。我们的分析揭示了一个集体的空间感知机制，这需要一个完整的反馈回路和一个不相干的前馈回路的顺序动作。积分反馈是由一个群体产生的扩散性化学物质的积累来实现的。这种积累与营养消耗相结合，决定了年轮开始的时间。非相干前馈实现的环成熟的速率和持续时间的域大小的相反的效果。这种机制强调了时序控制在实现鲁棒模式缩放中的作用，并为研究自然系统中的现象提供了新的视角。
Scale invariance refers to the maintenance of a constant ratio of developing organ size to body size. Though common, its underlying mechanisms remain poorly understood. Here we examined scaling in engineered E. coli that can form self-organized core-ring patterns in colonies. We found that the ring width exhibits perfect scale invariance to the colony size. Our analysis revealed a collective space-sensing mechanism, which entails sequential actions of an integral feedback loop and an incoherent feedforward loop. The integral feedback is implemented by the accumulation of a diffusive chemical produced by a colony. This accumulation, combined with nutrient consumption, sets the timing for ring initiation. The incoherent feedforward is implemented by the opposing effects of the domain size on the rate and duration of ring maturation. This mechanism emphasizes a role of timing control in achieving robust pattern scaling and provides a new perspective in examining the phenomenon in natural systems.