Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity
Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity
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DOI：
10.1111/j.1399-0039.2004.00314.x
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发表时间：
2004-11-01
期刊：
影响因子：
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通讯作者：
Sasazuki, T
中科院分区：
文献类型：
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作者：
Shichijo, S;Keicho, N;Sasazuki, T
为了确定高免疫原性的严重急性呼吸综合征冠状病毒（SARS-CoV）的表位肽能够诱导持久的免疫力，我们首先筛选免疫球蛋白G（IgG）抗体反应的197个不同的重叠15-聚体从三个感染患者的血清中的SARS-CoV蛋白。其中42种肽与所有三名患者的血清反应。因此，我们在感染后6个月测试了这42种肽对患者血清（n = 45）的反应性。在45名患者中，分别有23名（51%）、27名（60%）和19名（42%）感染后血清中可检测到对42种肽中的三种（S791、M207和N161）特异性的显著较高水平的IgG抗体。这三个肽，其持久的免疫识别，可以提供更好地了解SARS-CoV的免疫原性。
In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.