Independent recruitment of mediator and SAGA by the activator Met4
Independent recruitment of mediator and SAGA by the activator Met4
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DOI：
10.1128/mcb.26.8.3149-3163.2006
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发表时间：
2006-04-01
影响因子：
5.3
通讯作者：
Kuras, L
中科院分区：
文献类型：
--
作者：
Leroy, C;Cormier, L;Kuras, L
Mediator is a key RNA polymerase II (Pol II) cofactor in the regulation of eukaryotic gene expression. It is believed to function as a coactivator linking gene-specific activators to the basal Pol H initiation machinery. In support of this model, we provide evidence that Mediator serves in vivo as a coactivator for the yeast activator Met4, which controls the gene network responsible for the biosynthesis of sulfur-containing amino acids and S-adenosylmethionine. In addition, we show that SAGA (Spt-Ada-Gcn5-acetyltransferase) is also recruited to Met4 target promoters, where it participates in the recruitment of Pol II by a mechanism involving histone acetylation. Interestingly, we find that SAGA is not required for Mediator recruitment by Met4 and vice versa. Our results provide a novel example of functional interplay between Mediator and coactivators involved in histone modification.
