OP9 mouse stromal cells rapidly differentiate into adipocytes: characterization of a useful new model of adipogenesis
OP9 mouse stromal cells rapidly differentiate into adipocytes: characterization of a useful new model of adipogenesis
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DOI：
10.1194/jlr.d500037-jlr200
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发表时间：
2006-02-01
影响因子：
6.5
通讯作者：
Bickel, PE
中科院分区：
文献类型：
--
作者：
Wolins, NE;Quaynor, BK;Bickel, PE
从细胞培养模型中，尤其是从3T3 - L1细胞中，人们已经了解到很多关于脂肪细胞生物学的知识。3T3 - L1模型存在一些局限性，包括需要两周时间来生成脂肪细胞以及在培养过程中脂肪生成潜能的下降。我们研究了OP9细胞（一种源自骨髓的小鼠基质细胞系）重现脂肪生成的能力。当给予OP9细胞三种脂肪生成刺激中的任何一种时，它们会迅速积累三酰甘油，呈现脂肪细胞形态，并表达脂肪细胞晚期标记蛋白，包括葡萄糖转运蛋白4和脂联素。OP9细胞能在两天内分化为脂肪细胞。这种快速的分化速度使得在成熟的OP9脂肪细胞中检测瞬时表达的蛋白成为可能。OP9细胞中的脂肪生成涉及脂肪细胞分化的主要转录调节因子——过氧化物酶体增殖物激活受体γ（PPARγ）。OP9细胞是晚期前脂肪细胞，因为在添加脂肪生成刺激之前，它们就表达脂肪细胞蛋白CCAAT/增强子结合蛋白α和β、PPARγ、固醇调节元件结合蛋白 - 1、S3 - 12和 perilipin。在高细胞密度下培养或长时间连续培养，OP9细胞的分化能力不会减弱，这有利于生成保留脂肪生成潜能的稳定细胞系。因此，OP9细胞的独特特性将加速脂肪细胞生物学的研究。
Much knowledge of adipocyte biology has been learned from cell culture models, most notably 3T3-L1 cells. The 3T3-L1 model has several limitations, including the requirement of 2 weeks to generate adipocytes and the waning of adipogenic potential in culture. We have investigated the capacity of OP9 cells, a line of bone marrow-derived mouse stromal cells, to recapitulate adipogenesis. When OP9 cells are given any one of three adipogenic stimuli, they rapidly accumulate triacylglycerol, assume adipocyte morphology, and express adipocyte late marker proteins, including glucose transporter 4 and adiponectin. OP9 cells can differentiate into adipocytes within 2 days. This rapid rate of differentiation allows for the detection of transiently expressed proteins in mature OP9 adipocytes. Adipogenesis in OP9 cells involves the master transcriptional regulator of adipocyte differentiation, peroxisome proliferator-activated receptor gamma (PPAR gamma). OP9 cells are late preadipocytes in that, before the addition of adipogenic stimuli, they express the adipocyte proteins CCAAT/enhancer binding proteins a and B, PPARg, sterol-regulatory element binding protein-1, S3-12, and perilipin. OP9 differentiation is not diminished by maintenance in culture at high cell density or by long periods in continuous culture, thereby facilitating the generation of stable cell lines that retain adipogenic potential. Thus, the unique features of OP9 cells will expedite the study of adipocyte biology.