INTERACTION OF BCG-ACTIVATED MACROPHAGES WITH NEOPLASTIC AND NON-NEOPLASTIC CELL LINES INVITRO - QUANTITATION OF CYTOTOXIC REACTION BY RELEASE OF TRITIATED-THYMIDINE FROM PRELABELED TARGET-CELLS
INTERACTION OF BCG-ACTIVATED MACROPHAGES WITH NEOPLASTIC AND NON-NEOPLASTIC CELL LINES INVITRO - QUANTITATION OF CYTOTOXIC REACTION BY RELEASE OF TRITIATED-THYMIDINE FROM PRELABELED TARGET-CELLS
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DOI：
10.1093/jnci/54.5.1177
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
LEONARD, EJ
中科院分区：
文献类型：
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作者：
MELTZER, MS;TUCKER, RW;LEONARD, EJ
牛分枝杆菌BCG株感染IP小鼠的腹膜细胞在体外对同基因肿瘤细胞具有细胞毒作用。通过测量预先标记的靶细胞释放的氚-胸腺嘧啶核苷(~3H-TdR)来评价细胞毒性。负责肿瘤细胞毒性的细胞是巨噬细胞。来自未感染小鼠的巨噬细胞或来自油、淀粉或硫代乙二醇酸诱导的腹膜渗出物的巨噬细胞对标记的肿瘤单层几乎没有影响。抗肿瘤巨噬细胞在注射卡介苗后3-7天出现，并持续6周。两对肿瘤/非肿瘤细胞系，所有四种细胞系都来自克隆的同基因胚胎细胞系，被用作卡介苗激活的巨噬细胞的靶细胞。两种肿瘤细胞株的~3H-TdR释放量均显著高于两种非肿瘤细胞系。在肿瘤/非肿瘤混合靶细胞群中，卡介苗激活的巨噬细胞选择性地摧毁肿瘤细胞；非肿瘤细胞不受“无辜旁观者”的影响。
Peritoneal cells from mice infected ip withMycobacterium bovis, strain BCG, were cytotoxic to syngeneic tumor cells in vitro. Cytotoxicity was estimated by measurement of release of tritiated-thymidine (3H-TDR) from prelabeled target cells. The cell responsible for tumor cytotoxicity was the macrophage. Macrophages from uninfected mice or from oil-, starch-, or thioglycollateinduced peritoneal exudates had little effect on labeled tumor monolayers. Tumoricidal macrophages were present at 3–7 days and persisted through 6 weeks after a single BCG injection. Two neoplastic/nonneoplastic cell-line pairs, all four of the cell lines derived from a cloned syngeneic embryo cell line, were used as target cells for BCG-activated macrophages. Both tumor cell lines released significantly more3H-TDR than did the two nonneoplastic lines. In a mixed neoplastic/nonneoplastic target cell population, BCG-activated macrophages selectively destroyed the neoplastic cells; nonneoplastic cells were not affected as “innocent bystanders”.