EFFECT OF POLYMORPHONUCLEAR LEUKOCYTES ON SYNOVIAL CELL COLLAGENASE PRODUCTION
EFFECT OF POLYMORPHONUCLEAR LEUKOCYTES ON SYNOVIAL CELL COLLAGENASE PRODUCTION
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DOI：
10.2220/biomedres.3.506
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发表时间：
1982-01-01
影响因子：
1.2
通讯作者：
NAGAI, Y
中科院分区：
文献类型：
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作者：
HASHIDA, R;TERATO, K;NAGAI, Y
为了阐明炎症细胞在慢性炎性病变(如类风湿性关节炎和胶原性关节炎)的结缔组织破坏中的作用，在体外细胞培养系统中研究了[大鼠]多形核(PMN)白细胞对滑膜细胞的影响。腹膜PMN细胞提取液可显著诱导滑膜细胞胶原酶的产生。在含10%胎牛血清的德尔贝科‘S改良鹰’S培养液(D-MEM)中短时间培养的PMN细胞中也检测到该诱导因子。加入细胞松弛素B或外源颗粒可显著促进因子的释放，提示因子是通过胞吐作用从PMN细胞颗粒中释放出来的。该因子的相对分子质量估计约为20000道尔顿。在PMN细胞的胞浆组分中也发现了类似的刺激因子，它由两个分子大小组成，分别对应于20,000和45,000道尔顿，约占细胞总刺激活性的70%。PMN细胞不仅通过释放自身的组织降解酶，而且通过诱导滑膜细胞胶原酶的产生，明显参与了慢性炎症关节结缔组织的病理破坏。
To clarify the roles of inflammatory cells in connective tissue destruction at chronic inflammatory lesions, such as joints with rheumatoid arthritis and collagen-induced arthritis, the effects of [rat] polymorphonuclear (PMN) leukocytes on synovial cells were studied in an in vitro cell culture system. Extracts of peritoneal PMN cells significantly induced synovial cell collagenase production. This inducing factor was also detected in the medium after short-time culture of PMN cells in Dulbecco''s modified Eagle''s medium (D-MEM) containing 10% fetal calf serum (FCS). The release of the factor into the medium was markedly enhanced by the addition of cytochalasin B or foreign particles, suggesting that the factor is released from the granules of PMN cells by exocytosis. The MW of the factor was estimated to be approximately 20,000 daltons. Similar stimulating factors were detected in the cytosol fraction of PMN cells, which comprised 2 molecular sizes corresponding to 20,000 and 45,000 daltons and accounted for approximately 70% of total stimulating activity in the cell. PMN cells are evidently involved in the pathological destruction of the connective tissue at chronic inflammatory joints, not only by releasing their own tissue-degrading enzymes, but also by inducing synovial cell collagenase production.