Cloning and characterization of Hepp, a novel gene expressed preferentially in hematopoietic progenitors and mature blood cells
Cloning and characterization of Hepp, a novel gene expressed preferentially in hematopoietic progenitors and mature blood cells
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DOI：
10.1006/bcmd.2001.0434
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发表时间：
2001-05-01
影响因子：
2.3
通讯作者：
Jurecic, R
中科院分区：
文献类型：
--
作者：
Abdullah, JM;Jing, X;Jurecic, R
通过小鼠造血干细胞（HSC）和祖细胞消减cDNA文库的差异筛选，我们已经确定了一个祖细胞特异性转录，代表一种新的基因，命名为Hepp（造血祖细胞蛋白）。小鼠Hepp基因编码237个氨基酸的蛋白质，没有可检测的已知功能结构域或基序。缺乏无脊椎动物直系同源物和高度进化保守的肽序列在脊椎动物物种（斑马鱼，小鼠，人）表明，Hepp基因可能已经保守，但尚未未知的功能在脊椎动物。小鼠Hepp在成年组织中显示出有限的表达模式，并且在心脏、肺、脾和胸腺中以非常低的水平转录，而在肌肉中以较高的水平转录。在胚胎造血过程中，Hepp在小鼠胎肝HSC（Sca 1（+）c-kit（+）AA4.1（+）Lin（-）细胞）中不表达，但在造血祖细胞群体（AA4.1（-）细胞）中大量转录。类似地，在成人造血过程中，Hepp在骨髓HSC（Rh-123（低）Sca-1（+）c-kit（+）Lin（-）细胞）的高度富集群体中不转录，但其表达随着骨髓HSC（Lin（-）Sca-1（+）细胞）的更大异质群体分化成祖细胞（Lin（-）Sca-1（-）细胞）和更成熟的淋巴样和髓样细胞类型而上调。在成人组织中的有限表达模式和在胎儿和成人造血祖细胞和成熟血细胞中的优先表达表明，Hepp可能参与HSC和祖细胞谱系定型和分化的分子调控。(C)北京：科学出版社.
Through differential screening of mouse hematopoietic stem cell (HSC) and progenitor-subtracted cDNA libraries we have identified a progenitor cell-specific transcript that represents a novel gene, named Hepp (hematopoietic progenitor protein). The mouse Hepp gene encodes a protein of 237 amino acids with no detectable known functional domains or motifs. Lack of invertebrate orthologs and a high degree of evolutionary conservation of the peptide sequence in vertebrate species (zebrafish, mouse, human) suggest that the Hepp gene could have conserved although as yet unknown function in vertebrates. Mouse Hepp shows a restricted expression pattern in adult tissues and is transcribed at a very low level in heart, lung, spleen, and thymus and at a higher level in muscle. During embryonic hematopoiesis Hepp is not expressed in mouse fetal liver HSC (Sca1(+)c-kit(+)AA4.1(+)Lin(-) cells), but is abundantly transcribed in the population of hematopoietic progenitors (AA4.1(-) cells). Similarly, during adult hematopoiesis Hepp is not transcribed in the highly enriched population of bone marrow HSC (Rh-123(low)Sca-1(+)c-kit(+)Lin(-) cells), but its expression is upregulated as a greater heterogeneous population of bone marrow HSC (Lin(-)Sca-1(+) cells) differentiates into progenitors (Lin(-)Sca-1(-) cells) and more mature lymphoid and myeloid cell types. A restricted pattern of expression in adult tissues and preferential expression in both fetal and adult hematopoietic progenitors and mature blood cells suggest that Hepp could be involved in molecular regulation of HSC and progenitor cell lineage commitment and differentiation. (C) 2001 Academic Press.