CpG island methylator phenotype of multigene in serum of sporadic breast carcinoma
CpG island methylator phenotype of multigene in serum of sporadic breast carcinoma
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DOI：
10.1007/s13277-010-0040-x
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发表时间：
2010-08-01
期刊：
影响因子：
--
通讯作者：
Hu Lihua
中科院分区：
文献类型：
--
作者：
Feng Jing;Wang Yuping;Hu Lihua
CpG岛甲基化表型（CIMP）涉及靶向多个基因启动子的甲基化。我们测定了散发性乳腺癌（SBC）患者血清中肿瘤相关基因的甲基化谱。采用甲基化特异性聚合酶链反应检测50例SBC和50例配对非肿瘤患者血清中的多基因甲基化，CIMP+定义为有3个基因一致甲基化。50例SBC血清中10个基因的甲基化频率从FHIT的10%到RASSF1A的74%不等。RASSF1A、BRCA1、p16、CDH1、ER、RAR β 2、APC和DAPK的甲基化状态与SBC和非肿瘤血清显著相关（P
CpG island methylator phenotype (CIMP) involves methylation targeted toward the promoters of multiple genes. We determined a methylation profile of tumor-related genes in serum of sporadic breast cancer (SBC). The multigene methylation was examined by methylation-specific polymerase chain reaction assay in serum of 50 SBCs and 50 paired nontumors, and CIMP+ was defined as having three genes that are concordantly methylated. The methylation frequency of ten genes in serum of 50 SBCs varied from 10% in FHIT to 74% in RASSF1A. The methylation status of RASSF1A, BRCA1, p16, CDH1, ER, RAR beta 2, APC, and DAPK was significantly correlated with SBC and nontumor serum (P