Renal expression of alpha-smooth muscle actin and c-Met in children with Henoch-Schonlein purpura nephritis
Renal expression of alpha-smooth muscle actin and c-Met in children with Henoch-Schonlein purpura nephritis
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DOI：
10.1007/s00467-008-0749-6
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发表时间：
2008-06-01
影响因子：
3
通讯作者：
Hosoya, Mitsuaki
中科院分区：
文献类型：
--
作者：
Kawasaki, Yukihiko;Imaizumi, Tomoko;Hosoya, Mitsuaki
α-平滑肌肌动蛋白（α-SMA）是血管平滑肌细胞中占主导地位的肌动蛋白亚型，在纤维发生中起重要作用。另一方面，c-Met是肝细胞生长因子（HGF）的受体，其在保护免受损伤中起作用并具有抗纤维化作用。为了阐明α-SMA和HGF是否与过敏性紫癜性肾炎（HSPN）肾损伤的进展相关，我们评估了HSPN患者肾脏中α-SMA和c-Met的表达。患者分为三组。第1组包括8例儿童肾脏病国际研究（ISKDC）分类中II期或更低的患者（男：女4：4），第2组包括20例ISKDC III期或更高且预后良好的患者（男：女11：9），第3组包括7例ISKDC III期或更高且预后不良的患者（男：女3：4）。研究各组的肾活检结果，包括c-Met和α-SMA染色。首次活检时，第2组和第3组的肾脏α-SMA和肾小球c-Met平均评分高于第1组，而第2组和第3组之间的肾脏α-SMA和肾小球c-Met平均评分均无差异。在第二次活检时，第3组中肾脏α-SMA染色的平均评分高于第2组，第3组中肾小球c-Met染色的平均评分低于第2组。在第2组和第3组中，第一次活检时肾小球和间质α-SMA染色的平均评分与第二次活检时的慢性指数（CI）相关，但第一次活检时肾小球c-Met染色的平均评分与所有组中第一次或第二次活检的活动指数（AI）和CI均不相关。我们的研究结果表明，肾α-SMA的表达可能与HSPN肾损伤的进展。
Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. On the other hand, c-Met is the receptor for hepatocyte growth factor (HGF), which plays a role in protection from injury and has anti-fibrogenetic effects. To clarify whether alpha-SMA and HGF are associated with the progression of renal injury in Henoch-Schonlein purpura nephritis (HSPN), we evaluated the renal expression of alpha-SMA and c-Met in HSPN patients. Patients were divided into three groups. Group 1 consisted of eight patients (male:female 4:4) with stage II or less in the classification of the International Study of Kidney Disease in Children (ISKDC), Group 2 consisted of 20 patients (male:female 11:9) with ISKDC stage III or greater and a good prognosis, and group 3 consisted of seven patients (male:female 3:4) with ISKDC stage III or greater and poor prognosis. Renal biopsy findings, including c-Met and alpha-SMA staining, were investigated for each group. At first biopsy, the mean scores for renal alpha-SMA and glomerular c-Met in groups 2 and 3 were higher than those in group 1, while mean scores for neither renal alpha-SMA nor glomerular c-Met differed between groups 2 and 3. At second biopsy, the mean scores for renal alpha-SMA staining in group 3 were higher than those in group 2, and mean score for glomerular c-Met staining in group 3 was lower than that in group 2. In groups 2 and 3, the mean scores for glomerular and interstitial alpha-SMA staining at first biopsy were correlated with the chronicity index (CI) at second biopsy, but the mean score for glomerular c-Met staining at first biopsy correlated with neither the activity index (AI) nor CI in the first or second biopsies in all groups. Our findings suggest that the expression of renal alpha-SMA may be associated with progression of renal injury in HSPN.