Simultaneous on-chip surface plasmon resonance measurement of disease marker protein and small metabolite combined with immuno- and enzymatic reactions
Simultaneous on-chip surface plasmon resonance measurement of disease marker protein and small metabolite combined with immuno- and enzymatic reactions
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DOI：
10.1246/cl.2008.698
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发表时间：
2008-07-05
影响因子：
1.6
通讯作者：
Niwa, Osamu
中科院分区：
文献类型：
--
作者：
Nakamoto, Kohei;Kurita, Ryoji;Niwa, Osamu
采用便携式表面等离子体共振（SPR）系统和PDMS微通道同时检测疾病标记蛋白和校正标记蛋白。用抗体和含有辣根过氧化物酶（HRP）的氧化还原聚合物修饰微通道中的两层金膜。在上游固定化肌酐酶、肌酐酶和肌氨酸氧化酶。我们可以通过免疫反应和氧化还原反应引起的SPR角度偏移测量糖蛋白、转铁蛋白（MW: 75 kDa）和肌酐（MW: 113），表明单次注射尿转铁蛋白浓度可以与肌酐浓度归一化。
Disease marker protein and a correction marker were simultaneously measured with a portable surface plasmon resonance (SPR) system and PDMS microchannel. Two gold films in the microchannel were modified with antibody and redox polymer containing horseradish peroxidase (HRP). Creatininase, creatinase, and sarcosine oxidase were immobilized in the upstream. We could measure glycoprotein, transferrin (MW: 75 kDa) and creatinine(MW: 113) both by SPR angle shifts caused by immuno- and redox reactions, indicating that urinary transferrin concentration could be normalized with creatinine concentration by single injection.