BMP-2 and ALP gene expression induced by a BMP-2 gene–fibronectin–apatite composite layer
BMP-2 and ALP gene expression induced by a BMP-2 gene–fibronectin–apatite composite layer
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DOI：
10.1088/1748-6041/6/4/045004
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发表时间：
2011-08
影响因子：
4
通讯作者：
Xiupeng Wang;A. Oyane;H. Tsurushima;Y. Sogo;Xia Li;A. Ito
中科院分区：
文献类型：
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作者：
Xiupeng Wang;A. Oyane;H. Tsurushima;Y. Sogo;Xia Li;A. Ito
在羟基磷灰石陶瓷支架表面制备了具有基因-纤维连接蛋白(Fn) -磷灰石复合层的骨形态发生蛋白2 （BMP-2）基因传递系统。将BMP-2基因- Fn -磷灰石复合层涂覆在支架上，使用添加BMP-2 DNA和Fn的过饱和磷酸钙溶液。将支架异位植入大鼠背部皮下组织。植入4周后，包被BMP-2基因- fn -磷灰石复合层的羟基磷灰石支架与包被磷灰石层的支架相比，BMP-2基因和碱性磷酸酶的表达有所改善。虽然这些结果提示了基因传递系统诱导异位骨形成的可能性，但还需要进一步的研究来证明这一点。
The bone morphogenetic protein 2 (BMP-2) gene delivery system with a gene–fibronectin (Fn)–apatite composite layer was fabricated on the surface of a hydroxyapatite ceramic scaffold. The BMP-2 gene–Fn–apatite composite layer was coated on the scaffold using a supersaturated calcium phosphate solution supplemented with BMP-2 DNA and Fn. The scaffolds were ectopically implanted into the dorsal subcutaneous tissue of rats. Four weeks after the implantation, the hydroxyapatite scaffold coated with the BMP-2 gene–Fn–apatite composite layer showed improved gene expressions of BMP-2 and alkaline phosphatase as compared with the scaffold coated with the apatite layer. Although these results suggest the possibility of ectopic bone formation induced by the present gene delivery system, further study is necessary to prove this.