Chronic photodamage in skin of mast cell-deficient mice
Chronic photodamage in skin of mast cell-deficient mice
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DOI：
10.1111/j.1751-1097.1999.tb07996.x
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发表时间：
1999-08-01
影响因子：
3.3
通讯作者：
Kochevar, IE
中科院分区：
文献类型：
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作者：
Gonzalez, S;Moran, M;Kochevar, IE
日光性弹性组织变性是人类皮肤光老化的标志，也是实验性小鼠皮肤光老化的显著特征。肥大细胞的产物与光老化皮肤的发展有关。我们用肥大细胞缺陷小鼠WWv评价了肥大细胞产物是否介导UVB慢性诱导的小鼠皮肤变化。这些小鼠的反应与另一种白化病小鼠品系BALB/c的反应进行了比较。小鼠每周暴露于UVB辐射三次，持续11周;总剂量为18.8 J/cm（2），照射的WWv小鼠比照射的BALB/c小鼠表现出更大的表皮变化。在真皮中，弹性蛋白含量增加3.6倍，如锁链素所测量的，在WE处理的BALB/c小鼠中产生;相比之下，在UVB处理和未处理的WWV小鼠之间的弹性蛋白中没有观察到差异。两种品系的胶原蛋白含量均未因UVB处理而增加，而两种品系的UVB处理小鼠的糖胺聚糖含量增加量相似。与年龄匹配的未照射对照组相比，UVB治疗的BALB/c小鼠的肥大细胞数量增加了两倍，中性粒细胞数量增加了六倍。中性粒细胞，以及肥大细胞，在未经处理的和UVB处理的WWV小鼠皮肤缺席。这些结果表明，肥大细胞的产品是重要的，在小鼠皮肤日光性弹性组织变性的发展，无论是通过直接诱导弹性蛋白生产的成纤维细胞或间接介导的其他细胞类型的存在，产生的产品，增加成纤维细胞弹性蛋白的生产。
Solar elastosis is a hallmark of photoaged human skin and a prominent feature in experimentally produced photoaging in murine skin. The products of mast cells have been implicated in the development of photoaged skin. We evaluated whether products from mast cells mediate chronic UVB-induced changes in murine skin by employing a strain of mast cell-deficient mice, WWv, The responses in these mice were compared to those in BALB/c, another albino mouse strain. Mice were exposed three times per week to UVB radiation for 11 weeks; the total dose was 18.8 J/cm(2), Irradiated WWv mice showed greater epidermal alterations than the irradiated BALB/c mice. In the dermis, a 3.6-fold increase in elastin content, as measured by desmosine, was produced in the WE-treated BALB/c mice; in contrast, no difference was observed in elastin between UVB-treated and untreated WWv mice. Collagen content was not increased by UVB treatment in either strain, and the glycosaminoglycan content increased a similar amount in UVB-treated mice in both strains. The number of mast cells increased twofold and the number of neutrophils increased six-fold in UVB-treated BALB/c mice compared to age-matched unirradiated controls. Neutrophils, as well as mast cells, were absent in untreated and UVB-treated WWv mouse skin. These results suggest that products of mast cells are important in the development of solar elastosis in murine skin either by directly inducing elastin production by fibroblasts or indirectly by mediating the presence of other cell types that produce products that increase fibroblast elastin production.