The removal of human breast cancer cells from hematopoietic CD34+ stem cells by dielectrophoretic field-flow-fractionation
The removal of human breast cancer cells from hematopoietic CD34+ stem cells by dielectrophoretic field-flow-fractionation
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DOI：
10.1089/152581699319939
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发表时间：
1999-10-01
期刊：
影响因子：
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通讯作者：
Gascoyne, PRC
中科院分区：
文献类型：
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作者：
Huang, Y;Yang, J;Gascoyne, PRC
利用介电泳场流分离技术（DEP-FFF）从人乳腺癌MDA-435细胞中分离出造血CD 34（+）干细胞。利用排列在薄腔底部表面的叉指型微电极阵列产生介电泳力，使细胞混合物悬浮在流体流动剖面中。CD 34（+）干细胞悬浮得更高，被流体流携带得更快，并且比癌细胞更早地离开分离室。使用在线流式细胞术，在不到12分钟的时间内观察到细胞混合物的有效分离，并且获得纯度>99.2%的CD 34（+）干细胞级分。DEP-FFF的方法可能适用于许多生物医学细胞分离问题，包括微流体规模的诊断和细胞亚群的纯化。
Dielectrophoretic field-flow-fractionation (DEP-FFF) was used to purge human breast cancer MDA-435 cells from hematopoietic CD34(+) stem cells, An array of interdigitated microelectrodes lining the bottom surface of a thin chamber was used to generate dielectrophoretic forces that levitated the cell mixture in a fluid flow profile. CD34(+) stem cells were levitated higher, were carried faster by the fluid flow, and exited the separation chamber earlier than the cancer cells. Using on-line flow cytometry, efficient separation of the cell mixture was observed in less than 12 min, and CD34(+) stem cell fractions with a purity >99.2% were obtained. The method of DEP-FFF is potentially applicable to many biomedical cell separation problems, including microfluidic-scale diagnosis and preparative-scale purification of cell subpopulations.