CGRP and substance P in intraepithelial neuronal structures of the human upper respiratory system
CGRP and substance P in intraepithelial neuronal structures of the human upper respiratory system
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DOI：
10.1016/s0167-0115(97)01040-9
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发表时间：
1997-10-31
影响因子：
--
通讯作者：
Dietze, O
中科院分区：
文献类型：
--
作者：
Hauser-Kronberger, C;Hacker, GW;Dietze, O
应用免疫组化技术检测了人鼻黏膜和喉部表面及腺上皮上皮内上皮内神经纤维和神经内分泌细胞的分布。采用免疫荧光法和链霉亲和素-生物素-过氧化物酶复合物（S-ABC）法对神经元结构进行一般神经内分泌标记蛋白基因产物（PGP） 9.5和两种神经肽P物质（SP）和降钙素基因相关肽（CGRP）的免疫染色。具有游离神经末梢的上皮内神经纤维含有PGP 9.5，在鼻黏膜呼吸表面上皮和喉部鳞状上皮内发现。这些神经纤维亚群对SP和CGRP抗体表现出阳性免疫反应，在上皮下排泄管的导管上皮内检测到神经纤维通过基膜到达管腔部分。这些神经纤维表现出CGRP样的免疫反应性，但对SP没有反应性。在声门下和会声区检测到鳞状表面上皮内密集的神经纤维网络，其中含有少量的CGRP和SP神经纤维。会厌区单个上皮内味蕾和声门下上皮内神经内分泌细胞表达PGP 9.5。(C) 1997爱思唯尔科学有限公司
The distribution of intraepithelial nerve fibres and neuroendocrine cells within the surface and glandular epithelium of human nasal mucosa and larynx was examined using immunohistochemical techniques. Neuronal structures were immunostained for the general neuroendocrine marker protein gene-product (PGP) 9.5, and the two neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) using immunofluorescence and streptavidin-biotin-peroxidase complex (S-ABC) methods. Intraepithelial nerve fibres with free nerve endings contained PGP 9.5 and were found within the respiratory surface epithelium of the nasal mucosa and the squamous epithelium of the larynx. A subpopulation of these nerve fibres showed positive immunoreactivties with antibodies against SP and CGRP, Nerve fibres within the ductal epithelium of subepithelial excretory ducts passing the basal membrane and reaching the luminal part were detected. These nerve fibres showed CGRP-like immunoreactivity but not for SP. A dense network of nerve fibres within the squamous surface epithelium was detected in the subglottic and epiglottic region containing CGRP and SP in a small subpopulation of nerve fibres. Single intraepithelial taste buds in the epiglottic region and neuroendocrine cells within the subglottic epithelium expressed PGP 9.5. (C) 1997 Elsevier Science B.V.