Comprehensive Lipidome and Metabolome Profiling Investigations of Panax quinquefolius and Application in Different Growing Regions Using Liquid Chromatography Coupled with Mass Spectrometry
Comprehensive Lipidome and Metabolome Profiling Investigations of Panax quinquefolius and Application in Different Growing Regions Using Liquid Chromatography Coupled with Mass Spectrometry
复制标题
液相色谱-质谱联用技术对西洋参脂质组和代谢组的综合分析及其在不同产区的应用
DOI：
10.1021/acs.jafc.1c02241
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发表时间：
2021-06-03
影响因子：
6.1
通讯作者：
Wang, Xiao
中科院分区：
文献类型：
--
作者：
Li, Lili;Wang, Daijie;Wang, Xiao
西洋参是最知名的人参品种之一。本研究以甲醇/甲基叔丁基醚/水（0.3 mg/1 μ L/6 μ L/8 μ L）为提取溶剂，优化了西洋参脂质组和代谢物组的提取方法。共鉴定出497种代谢产物，包括365种脂类和76种皂甙。首先对西洋参进行了全面的脂质组分析，其中鉴定出32.6%的甘油磷脂、39.5%的甘油脂、9.3%的鞘脂、3.3%的甾醇脂质和15.3%的脂肪酰。正交偏最小二乘判别分析（OPLS-DA）结果表明，中国两个产区的代谢组学存在明显差异。在北方生长区，双层与非双层形成的膜脂（PC/PE，DGDGs/MGDGs），半乳糖脂中的酰基链的不饱和度，和膜甘油磷脂的含量增加。在东部种植区，贮藏脂、神经酰胺和脂肪酰的合成增加，次生代谢增强，发现24种差异皂苷，加深了对西洋参代谢调控机制的认识。
Panax quinquefolius is one of the most recognized ginseng species. In this study, lipidome and metabolome extraction methods for P. quinquefolius were optimized with methanol/methyl-tert-butyl ether/water (0.3 mg/1 mu L/6 mu L/8 mu L). A total of 497 metabolites were identified, including 365 lipids and 76 ginsenosides. Comprehensive lipidome profiling was first performed for P. quinquefolius, in which 32.6% glycerophospholipids, 39.5% glycerolipids, 9.3% sphingolipids, 3.3% sterol lipids, and 15.3% fatty acyls were identified. Orthogonal partial least squares discrimination analysis (OPLS-DA) showed obvious metabolomic differences in two growing regions of China. In the northern growing region, the ratio of bilayer- to nonbilayer-forming membrane lipids (PCs/PEs, DGDGs/MGDGs), the degree of unsaturation of acyl chains in galactolipids, and the content of membrane glycerophospholipids were increased. In the eastern growing region, the synthesis of storage lipids, ceramides, and fatty acyls was increased, and secondary metabolism was enhanced with 24 differential ginsenosides found. The investigation deepens the understanding of metabolic regulation mechanisms of P. quinquefolius.