Growth-phase-dependent expression of cspD, encoding a member of the CspA family in Escherichia coli
Growth-phase-dependent expression of cspD, encoding a member of the CspA family in Escherichia coli
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DOI：
10.1128/jb.179.16.5126-5130.1997
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发表时间：
1997-08
影响因子：
3.2
通讯作者：
Kunitoshi Yamanaka;Masayori Inouye
中科院分区：
文献类型：
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作者：
Kunitoshi Yamanaka;Masayori Inouye
大肠杆菌的cspD基因编码一种与冷休克蛋白CspA序列高度相似的蛋白，但cspD不受冷休克诱导表达。在本研究中，我们分析了cspD基因表达的调控。通过对cspD- lacz的融合和引物延伸分析，发现cspD的表达在静止生长状态下被显著诱导。然而，这种归纳不依赖于平稳相位sigma因子sigma。此外，cspD的表达与生长速率呈负相关，并受葡萄糖饥饿诱导。利用(p)ppGpp缺失菌株，我们发现(p)ppGpp是调节cspD表达的积极因子之一。
The cspD gene of Escherichia coli encodes a protein of high sequence similarity with the cold shock protein CspA, but cspD expression is not induced by cold shock. In this study, we analyzed the regulation of cspD gene expression. By using a cspD-lacZ fusion and primer extension analysis, the expression of cspD was found to be dramatically induced by stationary-phase growth. However, this induction does not depend on the stationary-phase sigma factor sigmaS. Moreover, the expression of cspD is inversely dependent on growth rates and induced upon glucose starvation. Using a (p)ppGpp-depleted strain, we found that (p)ppGpp is one of the positive factors for the regulation of cspD expression.