Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats.
Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats.
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DOI：
10.1016/j.foodchem.2020.127291
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发表时间：
2020-06
期刊：
影响因子：
8.8
通讯作者：
R. Thøgersen;J. Castro-Mejía;Ulrik Kræmer Sundekilde;Lars H Hansen;Nicola Gray;G. Kuhnle;Niklas Rye Jørgensen;A. Kornerup Hansen;Dennis Sandris Nielsen;H. C. Bertram
中科院分区：
文献类型：
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作者：
R. Thøgersen;J. Castro-Mejía;Ulrik Kræmer Sundekilde;Lars H Hansen;Nicola Gray;G. Kuhnle;Niklas Rye Jørgensen;A. Kornerup Hansen;Dennis Sandris Nielsen;H. C. Bertram
本研究调查了将菊粉和富含钙的牛奶矿物质掺入猪肉香肠中，以检查摄入后对胃肠道微生物组和生化活性的影响。给大鼠（n = 48）喂食四种香肠之一;富含1）菊粉（6.0%）和乳矿物质（3%），2）菊粉（6.0%），3）乳矿物质（3%）的猪肉香肠或4）未富集的对照香肠。基于NMR的代谢组学研究表明，菊粉富集增加了粪便中短链脂肪酸（SCFAs）的浓度。牛奶矿物质富集也增加了SCFA浓度，尽管不太明显。此外，牛奶矿物质降低了粪便和小肠内容物中亚硝基化合物的浓度。菊粉和牛奶矿物质的组合富集对SCFA的形成没有累积效应，并且似乎反对牛奶矿物质诱导的亚硝基化合物形成的减少。16 S rRNA基因扩增子测序表明，肠道微生物组的改变有助于观察到的效果。
This study investigated inulin and calcium-rich milk mineral incorporation into a pork sausage in order to examine the effects on microbiome and biochemical activity in the gastrointestinal tract upon ingestion. Rats (n = 48) were fed one of four sausages; a pork sausage enriched with 1) inulin (6.0%) and milk mineral (3%), 2) inulin (6.0%), 3) milk mineral (3%) or 4) control sausages without enrichment. NMR-based metabolomics revealed that inulin-enrichment increased the fecal concentration of short-chain fatty acids (SCFAs). Milk mineral-enrichment also increased SCFA concentrations, although less pronounced. In addition, milk mineral reduced the concentration of nitroso compounds in feces and small intestinal content. Combined enrichment with both inulin and milk mineral showed no cumulative effect on SCFA formation and seemed to oppose the milk mineral-induced reduction of nitroso compound formation. 16S rRNA gene amplicon sequencing indicated that alterations of the gut microbiome contributed to the observed effects.