Elevated Expression of follistatin Gene in the Pituitaries of Allotriploid Crucian Carp
Elevated Expression of follistatin Gene in the Pituitaries of Allotriploid Crucian Carp
复制标题
DOI：
10.1111/jwas.12499
复制
发表时间：
2018-04
影响因子：
2.8
通讯作者：
M. Tao;Shengnan Li;Can Song;Jie Chen;Hongjin Hu;Mi Luo;Rong Zhou;Shaojun Liu
中科院分区：
文献类型：
--
作者：
M. Tao;Shengnan Li;Can Song;Jie Chen;Hongjin Hu;Mi Luo;Rong Zhou;Shaojun Liu
卵泡抑素（Fst）作为一种旁分泌因子，在调节垂体促性腺激素（GTH）的合成与释放、性腺发育和排卵周期等方面发挥重要作用。本研究以二倍体红鲫（Carassius auratusred var.）获得异源三倍体鲫鲤和异源四倍体杂种。结果表明，三种鱼的olistatin基因均编码322个氨基酸。参照其他鱼类Fst蛋白的结构特征，发现这3种鱼的Fst蛋白分别具有4个结构域。逆转录聚合酶链反应（RT-PCR）结果表明，卵泡抑素基因在三种鱼体内除脾脏外的所有组织中均有表达。对不同季节概况的真实的实时定量PCR分析表明，异源三倍体在产卵前和产卵期的垂体中的奥利司他汀信使RNA表达显着更高。这些结果提示，异源三倍体垂体中卵泡抑素基因的高表达可能通过阻断激活素对促黄体生成素β亚基的抑制作用而导致异源三倍体不育。此外，研究结果有助于加深对三倍体生殖特性的认识，为多倍体育种提供参考。
As a paracrine factor, Follistatin (Fst) plays an important role in regulating biosynthesis and release of pituitary gonadotropin (GTH), gonadal development, and ovulation cycle. In this study, full‐length complementary DNAs (cDNAs) of thefollistatingene in diploid red crucian carp,Carassius auratusred var., allotriploid crucian carp, and allotetraploid hybrids were obtained. Our data showed that the cDNAs offollistatinof all three fish encoded 322 amino acids. Referring to the structural characteristics of Fst in other species, we found that the Fst protein of these three fish had four domains, respectively. Reverse transcriptase polymerase chain reaction (PCR) indicated that thefollistatingene was widely expressed in all tested tissues, except spleens, in these three fish. Real‐time quantitative PCR analysis of different seasonal profiles showed that allotriploids had significantly higher expression offollistatinmessenger RNA in pituitaries during both the prespawning and spawning periods. These results suggest that the elevated expression of thefollistatingene in the pituitaries of allotriploids might lead to sterility of allotriploids by blocking the inhibitory effect of activin on luteinizing hormone β subunit. Furthermore, the results may improve our understanding of reproduction characteristics in triploids, which benefits polyploidy breeding.