SNP and haplotype analysis reveal IGF2 variants associated with growth traits in Chinese Qinchuan cattle
SNP and haplotype analysis reveal IGF2 variants associated with growth traits in Chinese Qinchuan cattle
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DOI：
10.1007/s11033-013-2896-5
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Huang, Yong-Zhen;Zhan, Zhao-Yang;Chen, Hong
胰岛素样生长因子2（IGF 2）是一种有效的细胞生长和分化因子，与哺乳动物的生长和发育有关。本研究的目的是评估中国秦川牛IGF 2基因突变对生长性状的影响。采用DNA池测序和强制聚合酶链反应-限制性片段长度多态性（forcedPCR-RFLP）方法检测了牛IGF 2基因的4个单核苷酸多态性（SNPs）。我们还调查了单倍型结构和连锁不平衡（LD）系数的四个SNPs在817个人代表两个主要品种的牛从中国。单倍型分析结果表明，在研究群体中有8种不同的单倍型和27种组合基因型。统计分析表明，秦川牛群体的4个SNPs、组合基因型和单倍型与体高、体长、胸宽、胸深和体重相关（P < 0.05或< 0.01）。突变型变异体和突变单倍型（Hap 8：ATGG;可能是有益的QTN等位基因）是上级的生长性状;与野生型纯合子相比，杂合子双倍型与更高的生长性状相关。本研究结果为IGF 2基因多态性与生长性状相关提供了证据，并可用于肉牛育种的标记辅助选择。
Insulin-like growth factor 2 (IGF2) is a potent cell growth and differentiation factor and is implicated in mammals' growth and development. The objective of this study was to evaluate the effects of the mutations in the bovine IGF2 with growth traits in Chinese Qinchuan cattle. Four single nucleotide polymorphisms (SNPs) were detected of the bovine IGF2 by DNA pool sequencing and forced polymerase chain reaction-restriction fragment length polymorphism (forced PCR-RFLP) methods. We also investigated haplotype structure and linkage disequilibrium (LD) coefficients for four SNPs in 817 individuals representing two main cattle breeds from China. The result of haplotype analysis showed eight different haplotypes and 27 combined genotypes within the study population. The statistical analyses indicated that the four SNPs, combined genotypes and haplotypes are associated with the withers height, body length, chest breadth, chest depth and body weight in Qinchuan cattle population (P < 0.05 or < 0.01). The mutant-type variants and mutant haplotype (Hap 8: ATGG; likely to be the beneficial QTN allele) was superior for growth traits; the heterozygote diplotype was associated with higher growth traits compared to wild-type homozygote. Our results provide evidence that polymorphisms in the IGF2 gene are associated with growth traits, and may be used for marker-assisted selection in beef cattle breeding program.