Nanoparticle hydrophobicity dictates immune response.
Nanoparticle hydrophobicity dictates immune response.
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DOI：
10.1021/ja2108905
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发表时间：
2012-03-07
影响因子：
15
通讯作者：
Rotello, Vincent M
中科院分区：
文献类型：
--
作者：
Moyano, Daniel F;Goldsmith, Meir;Solfiell, David J;Landesman-Milo, Dalit;Miranda, Oscar R;Peer, Dan;Rotello, Vincent M
了解纳米材料与免疫系统的相互作用对于设计用于体内应用的新大分子系统至关重要。由于大多数大分子探针的复杂结构，免疫激活的系统研究具有挑战性。我们在这里提出了使用工程金纳米粒子，以确定疏水性对脾细胞的免疫反应的唯一影响。针对计算的纳米颗粒头基的LogP分析了一系列细胞因子（免疫报告子）的基因表达谱，在体外观察到免疫活性随着疏水性的增加而基本上线性增加。在体内小鼠模型中观察到一致的行为，证明了疏水性在免疫系统激活中的重要性。
Understanding the interactions of nanomaterials with the immune system is essential for the engineering of new macromolecular systems for in vivo applications. Systematic study of immune activation is challenging due to the complex structure of most macromolecular probes. We present here the use of engineered gold nanoparticles to determine the sole effect of hydrophobicity on the immune response of splenocytes. The gene expression profile of a range of cytokines (immunological reporters) was analyzed against the calculated LogP of the nanoparticle headgroups, with an essentially linear increase in immune activity with the increase in hydrophobicity observed in vitro. Consistent behavior was observed with in vivo mouse models, demonstrating the importance of hydrophobicity in immune system activation.