Sequence analysis of rabbit haemorrhagic disease virus (RHDV) in Australia: alterations after its release
Sequence analysis of rabbit haemorrhagic disease virus (RHDV) in Australia: alterations after its release
复制标题
DOI：
10.1007/s007050050490
复制
发表时间：
1999-01-01
影响因子：
2.7
通讯作者：
Cooke, BD
中科院分区：
文献类型：
--
作者：
Asgari, S;Hardy, JRE;Cooke, BD
用RT-PCR分析了1996年和1997年在澳大利亚五个州收集的被狂犬病病毒杀死的兔子的肝脏样本。用PCR扩增出一个398 bp的衣壳蛋白（VP60）基因片段，并对其进行直接测序。核苷酸和氨基酸序列的比对及其与澳大利亚释放的病毒原株的比较表明，两年后的遗传变化很小，同源性为98.2%至100%。构建的系统发育树表明，不同国家的核苷酸替换略有差异，但没有明确的证据表明，根据其地理起源的序列聚类。实际上，病毒RNA的测序提供了一种方法来测试病毒进一步释放和随后传播的效果，如果这种策略被用作加强RHD作为澳大利亚野兔生物控制的手段。
Liver samples from rabbits killed by RHDV, collected from five States in Australia in 1996 and 1997 were analysed by RT-PCR. A 398 bp fragment of the capsid protein (VP60) gene was amplified by PCR and directly sequenced. The alignment of the nucleotide and amino acid sequences and their comparison with the original strain of the virus released in Australia indicated genetic changes after two years have been small with 98.2% to 100% identity. The constructed phylogenetic tree suggests slight differences in nucleotide substitutions in various States but there is no clear evidence of clustering of sequences according to their geographic origin. In practical terms, sequencing of viral RNA provides a means of testing the efficacy of further releases and subsequent spread of the virus if such a strategy is employed as a means of enhancing RHD as a biological control of the wild rabbit in Australia.