Liquid chromatographic analysis of milk phospholipids with on-line pre-concentration
Liquid chromatographic analysis of milk phospholipids with on-line pre-concentration
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DOI：
10.1016/j.chroma.2004.04.051
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发表时间：
2004-10-29
影响因子：
4.1
通讯作者：
Wijesundera, C
中科院分区：
文献类型：
--
作者：
Fagan, P;Wijesundera, C
已经开发出两种通过 NP-HPLC 和蒸发光散射检测来分析牛乳磷脂 (PL) 类别的方法。在第一种方法中，使用PVA-Sil保护柱快速测定主要牛奶PL、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)和鞘磷脂(SM)。在第二种方法中，使用保护柱预浓缩 PL，然后通过柱切换阀将其在线转移到 PVA-Sil 分析柱上。这使得全乳 PL 得以分离，包括磷脂酰肌醇 (PI)、磷脂酰丝氨酸 (PS) 和溶血磷脂酰胆碱 (LPC)。 (C) 2004 Elsevier B.V. 保留所有权利。
Two methods have been developed for the analysis of bovine milk phospholipid (PL) classes by NP-HPLC with evaporative light scattering detection. In the first method, a PVA-Sil guard column was used for the rapid determination of the major milk PL, phosphatidylethathanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM). In the second method, the guard column was used to pre-concentrate the PL, which were then transferred on-line onto a PVA-Sil analytical column by the use of column switching valves. This enabled separation of complete milk PL, including phosphatidylinositol (PI), phosphatidylserine (PS) and lysophosphatidylcholine (LPC). (C) 2004 Elsevier B.V. All rights reserved.