Fast chromatin immunoprecipitation assay.
Fast chromatin immunoprecipitation assay.
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快速染色质免疫沉淀测定法。
DOI：
10.1093/nar/gnj004
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发表时间：
2006-01-05
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
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作者：
染色质免疫沉淀（ChIP）是一种广泛使用的方法来探索蛋白质和DNA之间的体内相互作用。ChIP检测需要几天才能完成，涉及几个试管转移，并使用苯酚-chlorophorm或旋转柱纯化DNA。传统的ChIP方法在处理多个样品时成为一个挑战。我们开发了一种高效快速的Chelex树脂基ChIP程序，可显著缩短检测时间，仅使用一个试管即可分离PCR准备好的DNA。该方法极大地促进了在一个实验中用几种抗体在许多时间点上探测染色质变化。
Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol–chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.