Development of microsatellite loci for the invasive weed Wedelia trilobata (Asteraceae).
Development of microsatellite loci for the invasive weed Wedelia trilobata (Asteraceae).
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DOI：
10.3732/ajb.1000327
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发表时间：
2010-11
影响因子：
3
通讯作者：
Wei Wu;R. Zhou;Hui Huang;X. Ge
中科院分区：
文献类型：
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作者：
Wei Wu;R. Zhou;Hui Huang;X. Ge
微卫星标记的开发是为了帮助阐明入侵物种三裂叶婆婆纳的群体遗传学。方法与结果采用FIASCO方法，筛选出23对扩增微卫星位点的引物，并对引物的重复顺序进行了分析。trilobata中有10个在两个群体中表现出多态性（每个位点2 ~ 5个等位基因）。trilobata，一个来自中国，一个来自秘鲁。其中6个基因座成功地从天然同源物W的样品中扩增出来。chinensis，具有预期的大小。结论这些标记可用于进一步研究三裂叶婆婆纳及其它近缘种的群体遗传学。
UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed to help elucidate the population genetics of the invasive species Wedelia trilobata. • METHODS AND RESULTS Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) repeats protocol, 23 sets of primers for amplifying microsatellite loci were identified in W. trilobata, 10 of which showed polymorphism (two to five alleles per locus) in samples of two populations of W. trilobata, one from China and one from Peru. Six of these loci were successfully amplified from samples of the native congener W. chinensis, with expected sizes. • CONCLUSIONS These markers may be useful for further investigation of population genetics of Wedelia trilobata and other congener species.