The SWI/SNF ATP-Dependent Chromatin Remodeling Complex in Arabidopsis Responds to Environmental Changes in Temperature-Dependent Manner
The SWI/SNF ATP-Dependent Chromatin Remodeling Complex in Arabidopsis Responds to Environmental Changes in Temperature-Dependent Manner
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DOI：
10.3390/ijms21030762
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Sarnowski, Tomasz J.
中科院分区：
文献类型：
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作者：
Gratkowska-Zmuda, Dominika M.;Kubala, Szymon;Sarnowski, Tomasz J.
SWI/SNF ATP 依赖性染色质重塑复合物 (CRC) 在真核生物的转录、细胞周期、DNA 复制、修复和激素信号传导调节中发挥重要作用。 SWI/SNF CRC 的核心由 SWI2/SNF2 型 ATP 酶、SNF5 和两个 SWI3 亚基组成，足以在体外执行核小体重塑。拟南芥基因组编码四个 SWI2/SNF2 ATP 酶、四个 SWI3、一个 SNF5 和两个 SWP73 亚基。核心 SWI/SNF 组件的基因在植物生长、胚胎发生和孢子体发育过程中具有关键但不完全重叠的作用。在这里，我们发现拟南芥 swi3c 突变体在较低温度下生长时表现出表型逆转，导致其胚胎、根发育和育性缺陷的部分恢复。我们的数据表明 swi3c 突变改变了几个参与低温反应的基因的表达。 ICE1、MYB15 和 CBF1 靶基因上含有 SWI3C 的 SWI/SNF CRC 的位置取决于温度条件，因此 swi3c 突变也会影响几个冷响应 (COR) 基因的转录。这些发现，加上 swi3c/ice1 双突变体的遗传分析和 swi3c 植物增强的耐冻性，表明 SWI/SNF CRC 有助于微调植物对不同温度条件的生长反应。
SWI/SNF ATP-dependent chromatin remodeling complexes (CRCs) play important roles in the regulation of transcription, cell cycle, DNA replication, repair, and hormone signaling in eukaryotes. The core of SWI/SNF CRCs composed of a SWI2/SNF2 type ATPase, a SNF5 and two of SWI3 subunits is sufficient for execution of nucleosome remodeling in vitro. The Arabidopsis genome encodes four SWI2/SNF2 ATPases, four SWI3, a single SNF5 and two SWP73 subunits. Genes of the core SWI/SNF components have critical but not fully overlapping roles during plant growth, embryogenesis, and sporophyte development. Here we show that the Arabidopsis swi3c mutant exhibits a phenotypic reversion when grown at lower temperature resulting in partial restoration of its embryo, root development and fertility defects. Our data indicates that the swi3c mutation alters the expression of several genes engaged in low temperature responses. The location of SWI3C-containing SWI/SNF CRCs on the ICE1, MYB15 and CBF1 target genes depends on the temperature conditions, and the swi3c mutation thus also influences the transcription of several cold-responsive (COR) genes. These findings, together with genetic analysis of swi3c/ice1 double mutant and enhanced freezing tolerance of swi3c plants illustrate that SWI/SNF CRCs contribute to fine-tuning of plant growth responses to different temperature regimes.