Transcription factors interact with RNA to regulate genes
Transcription factors interact with RNA to regulate genes
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DOI：
10.1016/j.molcel.2023.06.012
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发表时间：
2023-07-20
期刊：
影响因子：
16
通讯作者：
Young, Richard A.
中科院分区：
文献类型：
--
作者：
Oksuz, Ozgur;Henninger, Jonathan E.;Young, Richard A.
转录因子（TFs）协调基因表达程序，定义每个细胞的身份。规范TF通过两个结构域来实现这一点，一个结合特定的DNA序列，另一个结合蛋白质共激活因子或辅抑制因子。我们发现至少一半的tf也结合RNA，通过一个以前未被识别的结构域，其序列和功能特征类似于HIV转录激活子Tat的富含精氨酸的基序。RNA结合通过促进DNA、RNA和TF在染色质上的动态结合来促进TF的功能。TF-RNA相互作用是一个保守的特征，对脊椎动物的发育很重要，在疾病中被破坏。我们认为，结合DNA、RNA和蛋白质的能力是许多tf的一般特性，是其基因调控功能的基础。
Transcription factors (TFs) orchestrate the gene expression programs that define each cell's identity. The canonical TF accomplishes this with two domains, one that binds specific DNA sequences and the other that binds protein coactivators or corepressors. We find that at least half of TFs also bind RNA, doing so through a previously unrecognized domain with sequence and functional features analogous to the arginine-rich motif of the HIV transcriptional activator Tat. RNA binding contributes to TF function by promoting the dynamic association between DNA, RNA, and TF on chromatin. TF-RNA interactions are a conserved feature important for vertebrate development and disrupted in disease. We propose that the ability to bind DNA, RNA, and protein is a general property of many TFs and is fundamental to their gene regulatory function.