Adhesivity and motility of a zoonotic spirochete: Implications in host-dependent pathogenicity.
Adhesivity and motility of a zoonotic spirochete: Implications in host-dependent pathogenicity.
复制标题
人畜共患病螺旋体的粘附性和运动性：对宿主依赖性致病性的影响。
DOI：
10.1101/2020.02.11.944587
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Nakamura S
中科院分区：
文献类型：
--
作者：
Xu J;Koizumi N;Nakamura S
细菌运动对许多致病物种在入侵和/或传播过程中至关重要。螺旋体细菌钩端螺旋体在包括人类在内的多种哺乳动物中引起出血、黄疸和肾炎等症状。虽然丧失运动性会减弱螺旋体，但运动性依赖性致病性的机制尚不清楚。本文针对钩端螺旋体在液体中游动和在固体表面爬行的特点，通过致病性和非致病性钩端螺旋体感染不同种类的培养肾细胞，研究了钩端螺旋体在宿主组织中的动力学特性。我们发现，在致病性钩端螺旋体的情况下，更大比例的细菌附着在宿主细胞上，并使用爬行机制持续长距离移动。我们的研究结果将螺旋体病原体的动力学和运动学特征与它们的毒力联系起来。
Bacterial motility is crucial for many pathogenic species in the process of invasion and/or dissemination. The spirochete bacteria Leptospira spp. cause symptoms, such as hemorrhage, jaundice, and nephritis, in diverse mammals including humans. Although loss-of-motility attenuate the spirochete, the mechanism of the motility-dependent pathogenicity is unknown. Here, focusing on that Leptospira spp. swim in liquid and crawl on solid surfaces, we investigated the spirochetal dynamics on the host tissues by infecting cultured kidney cells from various species with pathogenic and nonpathogenic leptospires. We found that, in the case of the pathogenic leptospires, a larger fraction of bacteria attached to the host cells and persistently traveled long distances using the crawling mechanism. Our results associate the kinetics and kinematic features of the spirochetal pathogens with their virulence.