Enhanced release of soluble urokinase receptor by endothelial cells in contact with peripheral blood cells
Enhanced release of soluble urokinase receptor by endothelial cells in contact with peripheral blood cells
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DOI：
10.1016/s0014-5793(00)02312-7
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发表时间：
2000-12-15
期刊：
影响因子：
3.5
通讯作者：
Vaheri, A
中科院分区：
文献类型：
--
作者：
Mustjoki, S;Sidenius, N;Vaheri, A
细胞表面的尿激酶受体（uPAR）在细胞外蛋白水解、细胞迁移和粘附中起重要作用。可溶性uPAR（suPAR）最近在血浆中被发现，但其来源尚不清楚。我们的研究结果表明，未受刺激的血液单核细胞和内皮细胞可以释放suPAR和增强释放时，单核细胞或血小板与内皮细胞一起培养。没有细胞-细胞接触的共培养不能增强suPAR释放。我们还发现了suPAR片段，已知是有效的诱导剂的趋化性，在共培养生长培养基样品。两者合计，我们的研究结果表明，正常的血浆suPAR可以产生的内皮细胞和单核细胞和suPAR释放在细胞-细胞接触可能有调节作用，细胞粘附。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
The urokinase receptor (uPAR) on the cell surface plays an important role in extracellular proteolysis, cell migration and adhesion. Soluble uPAR (suPAR) has been recently discovered in plasma, but its origin is unclear. Our results now demonstrate that both unstimulated blood mononuclear and endothelial cells can release suPAR and that the release is enhanced when either mononuclear cells or thrombocytes are cultured together with endothelial cells. Co-culture without cell-cell contacts fails to enhance suPAR release. We also found suPAR fragments, known to be potent inducers of chemotaxis, in co-culture growth medium samples. Taken together, our results suggest that normal plasma suPAR can be produced by endothelial and mononuclear cells and that suPAR release in cell-cell contacts may have a regulatory role in cell adhesion. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.