Interactions between cells and titanium surfaces
Interactions between cells and titanium surfaces
复制标题
DOI：
10.1016/s1389-0344(02)00032-1
复制
发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Hildebrand, H
中科院分区：
文献类型：
--
作者：
Eisenbarth, E;Velten, D;Hildebrand, H
细胞和植入材料之间的相互作用由材料的表面结构和/或表面组成决定。在过去的几年里，钛及钛合金在许多临床应用中证明了它们相对于其他种植材料的优越性。这种主要行为是由致密的被动氧化层造成的，它在氧化介质中在几毫秒内形成。在2级纯钛表面制备了厚度为100 nm的二氧化钛薄膜，并以含钒量较高的钛合金(Ti1.5Al25V)为对照，制备了二氧化钛薄膜。通过热氧化和阳极氧化以及采用溶胶-凝胶法进行涂层制备。对生成的氧化层的结构和化学成分进行了表征。在细胞检测中(增殖、四甲基偶氮唑蓝、形态、肌动蛋白染色)检测细胞的反应。结果表明，溶胶-凝胶法制备的氧化钛层能够保护电池免受有毒合金元素的影响，电池反应只受较薄的氧化钛表面层的影响，而不受块体材料组成的影响。(C)2002年，爱思唯尔科学公司出版。
The interaction between cells and implant materials is determined by the surface structure and/or surface composition of the material. In the past years, titanium and titanium alloys have proved their superiority over other implant materials in many clinical applications. This predominant behaviour is caused by a dense passive oxide layer which forms within milliseconds in oxidizing media. Titanium dioxide layers of 100 nm thickness were produced on the surface of cp-titanium grade 2, and on an experimental alloy of high vanadium content (Ti1.5Al25V) as a harmful control. The layers were produced by thermal and anodic oxidation and by coating by means of the sol-gel process. The resulting oxide layers were characterized with respect of their structure and chemical composition. In cell tests (proliferation, MTT, morphology, actin staining), the reaction of the cells was examined. It was shown that the sol-gel-produced titanium oxide layer is able to shield the cells from toxic alloying elements, with the result that the cell reaction is influenced only by the thin titanium oxide surface layer and not by the composition of the bulk material. (C) 2002 Published by Elsevier Science B.V.