Optimization of extraction technology of Astragalus polysaccharides by response surface methodology and its effect on CD40
Optimization of extraction technology of Astragalus polysaccharides by response surface methodology and its effect on CD40
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响应面法优化黄芪多糖提取工艺及其对CD40的影响
DOI：
10.1016/j.carbpol.2009.06.018
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发表时间：
2009-11-17
影响因子：
11.2
通讯作者：
Zhang Xue-guang
中科院分区：
文献类型：
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作者：
Li Rui;Chen Wei-chang;Zhang Xue-guang
黄芪（Astragalus polysaccharides，AP）是一种辅助治疗肺癌、消化道癌、泌尿系统癌等多种癌症的中草药。最佳工艺条件为：提取时间3h，料液比4，粒度33.8目。在此优化条件下，AP的最大提取率为16.32%，与预测值吻合较好。采用高效液相色谱法分析其化学成分，并对其药理作用进行评价。结果表明，AP可明显降低胃癌大鼠CD40表达率和蛋白表达。(C)2009爱思唯尔有限公司保留所有权利。
Astragali is a herbal medicine used as an adjuvant extensively in the treatment of various cancers of lung, digestive tract, urinary system, etc. Statistics-based experimental designs were applied to optimize the extraction conditions for Astragalus polysaccharides (AP). The optimum conditions were found to be: the values of extraction time 3 h, ratio of liquid to solid 4 and particle size 33.8 mesh. Under these optimized conditions, the maximum AP extraction rate was 16.32 (%), which well matches with the predicted value. Furthermore, the chemical composition was analyzed with HPLC, and the pharmacological effect of the AP was evaluated. Results showed that AP could significantly decreased CD40 expression rate and protein expression in rats with stomach cancer. (C) 2009 Elsevier Ltd. All rights reserved.