Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.
Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.
复制标题
乙醇依赖性的诱导增加了大鼠脑中信号肽酶 mRNA 水平。
DOI：
10.1007/bf00944199
复制
发表时间：
1994
影响因子：
4.3
通讯作者：
Jacquet,R
中科院分区：
文献类型：
--
作者：
Signs,SA;Jacquet,R
差异北方印迹杂交被用作筛选工具，以确定定量响应乙醇依赖性诱导的mRNA。成年雄性大鼠连续4天重复接受高剂量乙醇给药。该方案导致对乙醇的耐受性和依赖性的发展。从乙醇依赖的大鼠脑中提取RNA，用于构建cDNA文库。一个cDNA被鉴定为与在乙醇处理期间大鼠脑中增加的mRNA杂交。cDNA序列分析表明，它在大鼠脑中识别的mRNA与编码犬信号肽酶18 kDa亚基的mRNA非常相似。在乙醇处理开始后48小时内，观察到大鼠信号肽酶mRNA在脑中增加近2倍。在处理期间，乙醇没有显著改变β-肌动蛋白mRNA水平。这些结果支持乙醇反应信号肽酶mRNA在大鼠脑中的存在。
Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This regimen resulted in the development of tolerance and dependence upon ethanol. RNA isolated from the ethanol-dependent rat brains was used to construct a cDNA library. One cDNA was identified that hybridized to a mRNA which increased in rat brain during the ethanol treatment. Sequence analysis of the cDNA indicated that it recognized a mRNA in rat brain which was very similar to that which encodes the 18 kDa subunit of canine signal peptidase. The rat signal peptidase mRNA was observed to increase in brain nearly 2-fold within 48 h after the initiation of ethanol treatment. Ethanol did not significantly alter β-actin mRNA levels during the treatment period. These results support the existence of an ethanol-responsive signal peptidase mRNA in rat brain.