Seed longevity in oilseed rape (Brassica napus L.) - genetic variation and QTL mapping

Seed longevity in oilseed rape (Brassica napus L.) - genetic variation and QTL mapping
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DOI:
10.1017/s1479262111000372
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发表时间:
2011-07-01
影响因子:
1.1
通讯作者:
Boerner, Andreas
Boerner, Andreas
中科院分区:
生物学4区
文献类型:
--
作者:
Nagel, Manuela;Rosenhauer, Maria;Boerner, Andreas

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虽然油菜已成为欧洲最重要的油料作物之一,但对其种子在长期储存条件下的生存力知之甚少。在这里,我们报告了一组42个加入在德国异位基因库在IPK,Gatersleben的油菜种子寿命进行检查。储存26年的加入之间的发芽比较表明,生活力部分是遗传决定的,因为它在42%和98%之间。试图通过对加倍单倍体的作图群体进行三次人工老化处理来确定生存力的遗传基础。在N6、N7、N8、N15、N16和N18 6条染色体上检测到数量性状位点(QTL)。将这些QTL的染色体位置与拟南芥中的同线区域进行比较,以探索哪些基因可能是长寿遗传变异的基础。
Although oilseed rape has become one of the most important oil crops in Europe, little is known regarding the viability of its seed under conditions of long-term storage. We report here an examination of oilseed rape seed longevity performed on a set of 42 accessions housed at the German ex situ genebank at IPK, Gatersleben. A comparison of germination between the accessions stored for 26 years showed that viability was in part genetically determined, since it ranged between 42 and 98%. An attempt was made to define the genetic basis of viability by subjecting a mapping population of doubled haploids to three artificial ageing treatments. Quantitative trait loci (QTL) were detected on six chromosomes: N6, N7, N8, N15, N16 and N18. The chromosomal locations of these QTL were compared with their syntenic regions in Arabidopsis thaliana in order to explore what genes might underlie genetic variation for longevity.