DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP
DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP
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DOI：
10.1093/aob/mcz135
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发表时间：
2019-11-27
期刊：
影响因子：
4.2
通讯作者：
Davies, Julia M.
中科院分区：
文献类型：
--
作者：
Matthus, Elsa;Sun, Jian;Davies, Julia M.
背景与目的胞外三磷酸腺苷调控植物的一系列功能，包括细胞存活、适应和跨王国相互作用。叶和根中细胞外ATP的关键功能可能涉及作为第二信使(钙信号)的胞内游离钙的增加。这里的主要目的是确定在多大程度上叶片和根的钙反应需要拟南芥的DORN1/P2K1胞外ATP受体。第二个目的是测试细胞外ATP是否能在根中产生钙波。方法综述了叶和根对细胞外ATP的反应，以确定它们可能与钙信号转导和DORN1/P2K1有关。用Aequorin测定了野生型和多年生植物的叶片和根细胞内钙含量对三磷酸腺苷的响应。利用绿色荧光蛋白估计DORN1/P2K1在根中的空间丰度。以表达GCaMP3的野生型根为材料，研究了胞外ATP诱导细胞内钙离子浓度升高的空间变异。叶签名仅部分依赖于DORN1/P2K1，而根签名完全依赖。DORN1/P2K1在根中的分布支持在顶端钙信号的产生中起关键作用。根的顶端和亚顶端的钙信号可能相互独立地运作，但顶端的钙增加可以驱动亚顶端的增加，这与钙波是一致的。结论DORN1可能支持一些与钙相关的反应，但它可能不是拟南芥细胞外ATP的唯一受体。根的顶端有由细胞外ATP触发的钙波的能力。
Background and Aims Extracellular ATP governs a range of plant functions, including cell viability, adaptation and cross-kingdom interactions. Key functions of extracellular ATP in leaves and roots may involve an increase in cytosolic free calcium as a second messenger ('calcium signature'). The main aim here was to determine to what extent leaf and root calcium responses require the DORN1/P2K1 extracellular ATP receptor in Arabidopsis thaliana. The second aim was to test whether extracellular ATP can generate a calcium wave in the root.Methods Leaf and root responses to extracellular ATP were reviewed for their possible links to calcium signalling and DORN1/P2K1. Leaves and roots of wild type and dorn1 plants were tested for cytosolic calcium increase in response to ATP, using aequorin. The spatial abundance of DORN1/P2K1 in the root was estimated using green fluorescent protein. Wild type roots expressing GCaMP3 were used to determine the spatial variation of cytosolic calcium increase in response to extracellular ATP.Key Results Leaf and root ATP-induced calcium signatures differed markedly. The leaf signature was only partially dependent on DORN1/P2K1, while the root signature was fully dependent. The distribution of DORN1/P2K1 in the root supports a key role in the generation of the apical calcium signature. Root apical and sub-apical calcium signatures may operate independently of each other but an apical calcium increase can drive a sub-apical increase, consistent with a calcium wave.Conclusion DORN1 could underpin several calcium-related responses but it may not be the only receptor for extracellular ATP in Arabidopsis. The root has the capacity for a calcium wave, triggered by extracellular ATP at the apex.