A rotating operant chamber for use with microdialysis.
A rotating operant chamber for use with microdialysis.
复制标题
用于微透析的旋转操作室。
DOI：
10.1016/j.jneumeth.2019.108387
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发表时间：
2019
影响因子：
3
通讯作者：
Weber,StephenG
中科院分区：
文献类型：
--
作者：
Degreef,Bart;Ngo,KhanhT;Jaquins-Gerstl,Andrea;Weber,StephenG
背景近年来，微渗析和化学分离用于定量分析的时间分辨率有所提高。更快的微透析测量方法的出现有望有助于清醒动物的行为研究。然而，清醒动物的微透析通常使用流体换向器(旋转器)。旋转的体积对测量的时间分辨率是不利的。新的方法动物可以被安置在旋转的笼子里，这样就不需要旋转了，但是旋转的操作室是不可用的。在这里，我们描述了一种具有微透析能力的旋转操作室的设计和构造。我们修改了一个旋转的笼子，在碗形笼子的侧面增加了操作行为测试组件。模块化的板载控制器便于操作组件/计算机通信。电池为控制器和可操作部件提供电力。电池和控制器与笼子一起旋转，控制器与计算机进行无线通信。结果旋转的操作室可以训练大鼠按照提示取回蔗糖颗粒。微渗析和在线高效液相色谱可用于在大鼠自由活动并与操作行为测试组件相互作用时每隔一分钟测量多巴胺。与现有方法(S)相比，我们不知道清醒动物在操作室内进行一分钟的多巴胺测量。结论旋转笼子的改装简单明了。当动物清醒、活动并与周围环境相互作用时，纹状体多巴胺的一分钟观察可以完成。
BackgroundRecently, the time resolution of microdialysis followed by a chemical separation for quantitative analysis has improved. The advent of faster microdialysis measurements promises to aid in behavioral research on awake animals. However, microdialysis with awake animals generally employs a fluidic commutator (swivel). The swivel's volume is inimical to the time resolution of the measurements.New methodAnimals can be housed in rotating cages so that the swivel is not required, but rotating operant chambers are not available. Here we describe the design and construction of a rotating operant chamber with microdialysis capability. We modified a rotating cage by adding operant behavior testing components to the side of the bowl-shaped cage. A modular on-board controller facilitates operant component/computer communication. A battery provides power to the controller and the operant components. The battery and controller rotate with the cage, and the controller communicates with the computer wirelessly.ResultsThe rotating operant chamber can be used to train a rat to retrieve a sucrose pellet following a cue. Microdialysis and online liquid chromatography can be used to measure dopamine at one minute intervals while the rat moves freely and interacts with operant behavior testing components.Comparison with existing method(s)We are not aware of one-minute dopamine measurements in awake animals in an operant chamber.ConclusionsRotating cage modifications are straightforward. One-minute observations of striatal dopamine can be accomplished while an animal is awake, moving, and interacting with its surroundings.